Imbalances in T Cell-Related Transcription Factors Among Patients with Hashimoto’s Thyroiditis
Journal Title: Sultan Qaboos University Medical Journal - Year 2017, Vol 17, Issue 2
Abstract
Objectives: Imbalances in effector T cell functioning have been associated with a number of autoimmune diseases, including Hashimoto’s thyroiditis (HT). Differentiation of effector T helper (T) 1, T2, T17 and regulatory T cell (Treg) lymphocytes is regulated by transcription factors, including T1-specifc T box (T-bet), GATA binding protein-3 (GATA3), retinoid-related orphan receptor (ROR)-α and forkhead box P3 (FOXP3). Tis study aimed to investigate T1/T2, T1/Treg, T2/Treg and T17/Treg balances at the level of these transcription factors. Methods: Tis study took place between October 2015 and August 2016. Peripheral blood mononuclear cells were collected from a control group of 40 healthy women recruited from the Zahedan University of Medical Sciences, Zahedan, Iran, and a patient group of 40 women with HT referred to the Hazrat Ali Asghar Hospital, Zahedan. Total ribonucleic acid extraction was performed and the gene expression of transcription factors was quantitated using a real-time polymerase chain reaction technique. Results: Expression of T-bet and GATA3 was signifcantly elevated, while FOXP3 expression was signifcantly diminished among HT patients in comparison with the controls (P = 0.03, 0.01 and 0.05, respectively). Expression of RORα was higher among HT patients, although this difference was not signifcant (P = 0.15). Expression of T-bet/FOXP3, GATA3/FOXP3 and RORα/FOXP3 ratios were increased among HT patients in comparison with the controls (P <0.02, <0.01 and <0.01, respectively). Conclusion: Tese results indicate that HT patients have imbalances in T1/Treg, T2/Treg and T17/Treg lymphocytes at the level of the transcription factors, deviating towards T1, T2 and T17 cells. Correction of these imbalances may therefore be therapeutic.
Authors and Affiliations
Vahid Safdari| Physiology Research Center, Institute of Neuropharmacology and Department of Laboratory Sciences, School of Para-Medicine, Kerman University of Medical Sciences, Kerman, Iran, Ebrahim Alijani| Department of Immunology, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran, Maryam Nemati| Department of Laboratory Sciences, School of Para-Medicine, Kerman University of Medical Sciences, Kerman, Iran, Abdollah Jafarzadeh| Molecular Medicine Research Center and Department of Immunology, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
Keywords
Related Articles
- EP ID EP14770
- DOI 10.18295/squmj.2016.17.02.007
- Views 315
- Downloads 22
Sent to Explore, Conquer and Heal History of the evolution of biomedicine in Oman during the 19th century
During the past four decades, Oman has transformed into a modern state with remarkable changes in all felds, including public health and the provision of medical services. Little attention has been paid so far to the h...
Rate and Predictors of Publication of Resident Abstracts Presented at Oman Medical Specialty Board Scientific Meetings
Objectives: This study aimed to examine the rate and factors associated with the publication of abstracts presented by residents at Oman Medical Specialty Board (OMSB) scientific meetings. Methods: This retrospective stu...
Why the Economic Aspects of Healthcare are not Unique
Frequent claims suggest that healthcare and its production are not only different from other goods, but that they differ to such an extent that healthcare should be viewed as unique. Various features of healthcare, such...
Clinical Development of Experimental Therapies for Malignant Glioma
Advances in medical and surgical treatments in the last two to three decades have resulted in quantum leaps in the overall survival of patients with many types of non-central nervous system (CNS) malignant disease, whi...
The Effect of Change in Posture on Spirometry in Patients with Obstructive Sleep Apnoea Syndrome
Objectives: Obstructive sleep apnoea syndrome (OSAS) is a growing health concern as it is associated with serious comorbidities. OSAS is mainly related to obesity, age, gender and a narrowed upper airway is commonly seen...