Biogenic Synthesis,Characterization of Silver Nanoparticles using Tabernaemontana Divaricata and Polianthes Tuberosa Flower Extract and Evaluation of Their Antibacterial Activities
Journal Title: International Journal for Research in Applied Science and Engineering Technology (IJRASET) - Year 2014, Vol 2, Issue 8
Abstract
The importance of Tabernaemontana divaricata ,Polianthes tuberosa flower and Silver nanoparticles as revealed by various literature resources, we planned to carry out biogenic synthesis of Silver nanoparticles using the above two extracts. Silver nanoparticles were prepared by adopting standard procedure. The formations of Silver nanoparticles from the extracts were identified first by observing the colour changes. The extract colour changes during the formation of Silver nanoparticles from light yellow to yellowish-brown for Tabernaemontana divaricata and pale yellow to dark red for Polianthes tuberosa. Silver nanoparticle formations were characterized by UV, FT-IR, XRD and SEM. UV absorbance at 460nm and at 430nm for Silver nanoparticles derived from the above two flower extracts. FT-IR stretching frequencies at 509cm-1 for both the flower extracts proved the formation of silver nanoparticles. XRD & SEM analysis of silver nanoparticles indicated that they exist in spherical, face centered cubic (fcc) crystalline structure with size range 32nm and 17nm respectively. On comparing invitro bioactivities of Tabernaemontana divaricata flower extract , Polianthes tuberosa flower extract and their Silver nanoparticles by zone of inhibition studies showed that Silver nanoparticles are highly active against Staphylococcus aureus and Pseudomonas aeruginosa with the zone of inhibition in 16&11mm and 14&10mm respectively.
Authors and Affiliations
Valli. G, Perlina. R & Anusuya. M
Keywords
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