Extracellular Lipolytic Enzyme Production by a Novel Extremely Halophilic Bacterium
Journal Title: Universal Journal of Environmental Research and Technology - Year 2012, Vol 2, Issue 3
Abstract
Halophilic bacteria live in extreme salt conditions and can produce enzymes which are highly stable. A study was conducted to isolate lipolytic enzyme producing halophilic bacteria from salted fish. As lipolytic enzymes from halophiles can tolerate extreme salt conditions, it is highly stable and can tolerate harsh industrial conditions and thus has got potential biotechnological applications. In the present work 17 halophilic isolates were obtained from salted fish samples on Seghals Gibbons Complex (SGC) medium. The lipolytic activity of the strains was tested by Tween hydrolysis on SGC medium containing 1% Tween 80 or Tween 40. A strain SH1 which showed the highest relative enzyme activity was identified by 16 S rDNA sequencing as a novel strain belonging to the class Gamma proteobacteria. The strain was found to grow in a salt range, 2 to 5 M and thus confirmed as an extreme halophile. It showed lipolytic activity at salt concentration in the range 2 to 4 M and pH range 6 to 11. Thus it forms a promising strain for the production of halophilic lipolytic enzymes and thus can have industrial applications.
Authors and Affiliations
Anisha C, Jyothis Mathew and Radhakrishnan E K
Keywords
Related Articles
- EP ID EP31722
- DOI -
- Views 352
- Downloads 2
Evaluation of the Reproductive Potential of Bivoltine Silkworm Hybrids of Bombyx Mori L under High Temperature and High Humidity and High Temperature and Low Humidity Conditions of the Tropics
The challenging task of breeders is to develop silkworm breeds which can produce eggs under prevailing conditions of temperature and humidity. The present study was undertaken to evaluate the reproductive potential of...
Investigation on Ecological Parameters of Dyeing Organic Cotton Knitted Fabrics
In this study, the ecological parameters of the effluent obtained from dyeing of scoured and mercerised organic cotton single jersey fabrics with textile reactive dyes adopting exhaust dyeing method was investigated. Th...
Cotton Dyeing with Natural Dye Extracted from Pomegranate (Punica granatum) Peel
Use of natural dyes has increased several folds in the past few years due to the eco-friendly approach of the people. This paper concerns with the purification of natural dyestuff extracted from an abundantly occurring...
A Mini Review on Metagenomics and its Implications in Ecological and Environmental Biotechnology
Metagenomics is an applied science that deals with throughput analysis of Environmental Genomic isolates. Advances in genomic sequencing technologies such as parallel sequencing and sequence analysis methods have contr...
Solar Insolation at Chennai during the 23rd Solar cycle
The Solar irradiance is treated as a constant (solar constant) for many practical purposes. But, actually it varies by a small amount due to various causes. The objective of this paper is to emphasize the variations pro...