Lipopolysaccharide tolerance attenuates inflammatory responses by increasing hemeoxygenase 1 and tristetraprolin expression in Raw264.7 macrophages

Lipopolysaccharide tolerance attenuates inflammatory responses by increasing hemeoxygenase 1 and tristetraprolin expression in Raw264.7 macrophages

Journal

Subject and more

  • LCC Subject Category: Biological Sciences, Genetics, Microbiology, Pharmacology, Toxicology
  • Publisher's keywords: LPS tolerance, anti-inflammatory effects, HO1, TTP
  • Language of fulltext: english
  • Full-text formats available: PDF

AUTHORS

    Akhi Moni

EDITORIAL INFORMATION

FULL TEXT

To download PDF files Login to your Account.

ABSTRACT

Inflammation is associated with the progression of a variety of diseases. Lipopolysaccharides (LPS) tolerance is recognized to reduce proinflammatory responses. Heme oxygenase1 (HO1) and tristetraprolin (TTP) are induced by LPS tolerance and mediate the antiinflammatory effects. However, it was not clear whether two molecules are linked in LPS tolerance. In this study, we sought to determine whether HO1 associates with TTP to mediate the anti-inflammatory effects of LPS tolerance. LPS treatment significantly increased mRNA and protein level of HO1 and TTP in a time dependant fashion while LPS significantly decreased mRNA and protein level of TNFα in Raw264.7 macrophages. LPS tolerance inhibited TNFα mRNA and protein while HO1 and TTP level was still increased. In HO1 deficient macrophages, LPS tolerance failed to attenuate TNFα mRNA expression but TTP level was still decreased. Our results suggest that HO1 and TTP are functionally linked in mediating anti-inflammatory effects of LPS tolerance. This novel LPS tolerance-HO1-TTP signaling pathway provides new possibilities for the treatment of inflammatory diseases.

About Europub

EuroPub is a comprehensive, multipurpose database covering scholarly literature, with indexed records from active, authoritative journals, and indexes articles from journals all over the world. The result is an exhaustive database that assists research in every field. Easy access to a vast database at one place, reduces searching and data reviewing time considerably and helps authors in preparing new articles to a great extent. EuroPub aims at increasing the visibility of open access scholarly journals, thereby promoting their increased usage and impact.