Spectrochemical characterization of Vero cell line against PPR virus infection
Journal Title: Journal of Advanced Biotechnology and Experimental Therapeutics - Year 2019, Vol 2, Issue 1
Abstract
An in vitro virus infectivity assay based on the newly introduced spectrochemical method was performed using Peste des Petits Ruminants (PPR) virus infected Vero cell line as a model infection. Herein, the mitochondrial reductase enzyme activity was monitored using 3-(4,5-Dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) reagent as a substrate which was reduced to a insoluble formazan products. The purple to darker color was achieved upon hydrolysis of the insoluble formazan with DMSO solution. The absorbance (OD values) of colored solution was measured by spectrophotometer at a wave length of 570nm. The infectivity/viability data were achieved from the OD values of different doses of virus infected or non-infected cells using the standard formulae. The OD values obtained from spectrochemical assay were compared with traditional Plaque assay and validated with Trypan blue assay. The data obtained from spectrochemical analysis showed similar trend as was achieved with traditional methods with a little variation in the sensitivity. The sensitivity variations are obvious due to the marked differences in the measurement unit and detection methods. However the newly introduced spectrochemical method showed superiority over the traditional methods because of its simple, label free, less time consuming measurement method and its suitability in the monitoring of large number samples.
Authors and Affiliations
Md. Mizanur Rahman, Kumar Jyotirmoy Roy, Mst. Khudishta Aktar, Md. Rafiqul Islam, Md. Abdul Kafi
A brief review on Japanese Encephalitis in Swine with its diagnostic strategies
Japanese encephalitis is a viral disease affecting humans, mostly the children and is a major concern to public health. The single stranded flavivirus is mainly transmitted by mosquitoes of the Culex genus and causes n...
Antibacterial and cytotoxic activity of seeds of white hyacinth bean (Lablab purpureus L. sweet ‘white’)
Seeds of white hyacinth bean available in Khulna, Bangladesh were investigated to assess bioactivities such as antibacterial and cytotoxic properties. For the evaluation of bioactivities solvent extraction was performe...
Expression patterns of the phosphoproteins and total proteins in TLQP-21 (a VGF derived peptide) treated SH-SY5Y cells
VGF (non-acronymic), belonging to a large granin family, gives rise to a number of bioactive peptides by proteolysis and exert an extensive array of biological effects on energy metabolism, pain modulation, gastric sec...
Lipopolysaccharide tolerance attenuates inflammatory responses by increasing hemeoxygenase 1 and tristetraprolin expression in Raw264.7 macrophages
Inflammation is associated with the progression of a variety of diseases. Lipopolysaccharides (LPS) tolerance is recognized to reduce proinflammatory responses. Heme oxygenase1 (HO1) and tristetraprolin (TTP) are induc...
Prophylactic effects of vitamin E and selenium on di (n-butyl) phthalate-induced testicular damage in prepubertal rats
Di (n-butyl) phthalate (DBP), a potential endocrine disruptor, adversely affects male reproductive function via activation of oxidative stress. This study was conducted to investigate the protective effects of vitamin...