Insilico analysis of γ- aminobutyric acid transaminase (GABA-T) of Brassica napus (Rape)
Journal Title: Journal of Advanced Biotechnology and Experimental Therapeutics - Year 2019, Vol 2, Issue 1
Abstract
γ- aminobutyric acid (GABA) is an essential metabolite which plays a crucial role in signal transmission, stress metabolism, and some other activities also reported. Although the actual function of GABA shunt is not clearly understood. Three key enzymes, gamma aminobutyric acid transaminase (GABA-T), succinic semialdehyde dehydrogenase and succinic semialdehyde reductase are involved in GABA shunt mechanism en route from glutamate to the tricarboxylic acid cycle (TCA)which could pave the way of GABA shunt action. The enzyme gamma aminobutyric acid transaminase (GABA-T) could also play a key role in GABA shunt action by converting GABA to succinic semialdehyde (SSA).In this study, the protein sequence of γ-aminobutyric acid transaminase of Brassica napus (Rape) was retrieved from UniProt protein database and analyzed GABA-T enzyme using different bioinformatics tools and servers to analyze the physiochemical properties, amino acid composition, conformational states, and 3D structure. We found that our experimental protein sequence was very unstable, and the graph of Local Quality Estimate showed that the sequence was porn to mutation and value of Z score was above two in comparison with a non-redundant set of PDB structure. In addition, the phylogenetic tree revealed that GABA-T of Dendrobium officinale, Phoenix dactylifera, Oryza sativa, Arabidopsis thaliana and Brassica napus evolved from a common ancestor gene.
Authors and Affiliations
Shahidur Rahman and Md. Shahjahan
Keywords
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