INVESTIGATION OF SEED GERMINATION IN THE TRANSGENIC CANOLA (BRASSICA NAPUS L.) UNDER SALT STRESS
Journal Title: International Research Journal of Applied and Basic Sciences - Year 2011, Vol 2, Issue 9
Abstract
Abiotic stresses such as drought and salinity are the most considerable cause of crop productivity decrease in agriculture. Genetic engineering can improve crop tolerance to abiotic stresses by many ways. Mannitol is one of plant osmoprotectants to cope with osmotic stress. In the previous study, we developed transgenic Canola (Brassica napus L.) using bacterial mtlD gene. Here, salt stress tolerance of transgenic (+mtlD) and non-transgenic (-mtlD canola plants studied on seed germination stages. The sterilized transgenic and non-transgenic seeds were cultured on MS media (pH 5.8) at different concentrations of NaCl (0 250 300, 350, 400 and 450 mM). HPLC analysis results showed that transgenic plants accumulate mannitol on average of 0.0440 mg/gDW, whereas wild-type plants did not accumulate any mannitol. However, transgenic seeds germinated at 350 mM NaCl, non-transgenic plants germinated not more than 250 mM of NaCl. Therefore, we demonstrated that the mtlD gene was expressed in transgeniccanola plants, resulting in mannitol accumulationand improved salt tolerance in transgenic seeds.
Authors and Affiliations
ROYA MOTALLEBI| Tissue Culture and Gene Transformation Department, Agricultural Biotechnology Research Institute of Iran (ABRII), Mahdasht Road, P. O. Box 31535-1897, Karaj, Iran, HASSAN RAHNAMA*| Tissue Culture and Gene Transformation Department, Agricultural Biotechnology Research Institute of Iran (ABRII), Mahdasht Road, P. O. Box 31535-1897, Karaj, Iran
Effect Of Potasium Stress On Seed Germination And Growth Of Popcorn (K.Sc.6o4P.C)
Environmental factors may influence popcorn germination rates and seedling establishment before seeds are even produced. Evaporation and transpiration remove pure water from the soil. This water loss concentrates solutes...
Survey effectiveness of play therapy Is based on CBT on reduction behavior of children with ADHD
This study examined the effectiveness of CBT-based play therapy on symptom reduction in children with ADHD. Research 30 children who were randomly selected and randomly assigned to two groups of 15 control and tested wer...
The Effect of Vitamin "E" On the Peripheral Neurotoxicity of Antimony in Adult Male Albino Rat
The present work was planned with the aim to study the histological changes that might occur in the sciatic nerve of adult male albino rat following antimony trioxide exposure and to throw more light on the protective ro...
An Investigation on Effective Factors on Share of Agricultural Sector in GDP of Iranian Economy
This research analyzes how production main factors and relative prices affect agricultural sector share in gross domestic production. Subordinate features of gross domestic production, based on Dixit and Norman’s theatri...
A multiple response analysis: effective strategies toward reduce the gardeners problems in north of Iran
This study was conducted in order to find out the effective strategies for obviation of gardeners problems in north of Iran. This study was carried out by descriptive survey at March 2011. Target population was formed fr...