Molecular and cytological evaluation of male sterile and restorer lines in hybrids rice
Journal Title: International Research Journal of Applied and Basic Sciences - Year 2012, Vol 3, Issue 1
Abstract
Information on the genetics of fertility restoration facilitates breeding and/or selection of restorer lines used in hybrid breeding program in a cytoplasmic male sterility (CMS) system. Cytoplasmic male sterility (CMS) is a common phenomenon that has been extensively used for production of hybrid seeds in various crops. Rf genes are needed for restoring fertility to CMS lines. Searching for molecular tagging of restorer genes is of high importance where phenotyping is very time consuming and requires the determination of spikelet sterility in testcross progeny. In order to determine maintainers and fertility restorers lines in hybrids rice, 5 cytoplasmic male sterile was crossed whit 10 pure line as tester. In other year, F1 progenies arranged in RCBD design whit three Replications. Evaluation of fertility in F1 populations showed IR-9 parent for all the crosses was identified as restorer. The cross Rome parent with CMS lines observed lowest amount fertility, also molecular analysis confirmed exist gene RF only in IR-9 parent.
Authors and Affiliations
Ammar Gholizadeh ghara*| M. Sc student, Science and Research branch, Islamic Azad University, Tehran, Iran.Ammar Gholizadeh Ghara, Email: ammar_gholizadeh@yahoo.com, Ghorbanali Nematzadeh| Department of Agronomy and Plant Breeding, Genetics and Agricultural Biotechnology Institute of Tabarestan, Sari Agricultural Science and Natural Resources University, Sari, Iran, Nadali Bagheri| Department of Agronomy and Plant Breeding, Genetics and Agricultural Biotechnology Institute of Tabarestan, Sari Agricultural Science and Natural Resources University, Sari, Iran, Asa Ebrahimi| Assistant Professor of Biotechnology, Science and Research branch, Islamic Azad University, Tehran, Iran., Morteza Oladi| Department of Agronomy and Plant Breeding, Genetics and Agricultural Biotechnology Institute of Tabarestan, Sari Agricultural Science and Natural Resources University, Sari, Iran
Keywords
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