Journal of Herbal Drugs

Journal of Herbal Drugs

Basic info

  • Publisher: Islamic Azad University, Shahrekord Branch
  • Country of publisher: iran, islamic republic of
  • Date added to EuroPub: 2018/Feb/04

Subject and more

  • LCC Subject Category: Marketing, Agricultural Science, Biotechnology, Botany, Genetics, Plant Sciences, Medicine, Pharmacology, Toxicology
  • Publisher's keywords: Herbs, Cultivation or Agronomy, Breeding, Biotechnology, Marketing and Economy, Medicinal Plants, Genetic
  • Language of fulltext: english, persian
  • Full-text formats available: PDF

Publication charges

  • Article Processing Charges (APCs): No
  • Submission charges: No
  • Waiver policy for charges? No

Editorial information

Open access & licensing

  • Type of License: CC BY
  • License terms
  • Open Access Statement: Yes
  • Year open access content began: 2011
  • Does the author retain unrestricted copyright? False
  • Does the author retain publishing rights? False

Best practice polices

  • Permanent article identifier: DOI
  • Content digitally archived in: Nopolicy
  • Deposit policy registered in: None

This journal has '260' articles

Effect of Bakhtiari celery (Kelussia odoratissma Mozaff) on sensory properties and shelf life of set yogurt

Effect of Bakhtiari celery (Kelussia odoratissma Mozaff) on sensory properties and shelf life of set yogurt

Authors: Amir Shakerian; Mohammad-Javad Sohrabi; Abdollah Ghasemi Pirbalouti
( 16 downloads)
Abstract

Background & Aim: The essential oils are the most important secondary aromatic biochemical compounds in plants and even in some animals. They are of great importance because of their aromatic, flavoring and preventing properties from deterioration in food industries. According to ethnobotany study of aerial parts of Bakhtiari celery or Kelus (Kelussia odoratissma Mozaffarian) in dairy such as yogurt and buttermilk, encouraged us to do this research. Experimental: In this study, the essential oil of K. odoratissima Mozaff was extracted. Evolution was made of three concentrations of essential oil (20, 40 and 60 ppm) which added to yogurt after inoculation. The yogurt made by industrial method. During 30 days with certain intervals physical, chemical and microbial properties were analyzed. Results & Discussion: The results showed that essential oil of K. odoratissima Mozaff has improved significantly physical and chemical properties of yogurt. It led to increasing sensory properties, acidity control, improving flavor of yogurt and increasing time of consumption but water holding capacity (WHC) was reduced.

Keywords: Essential oil Kelussia odoratissima Mozaffarian Yogurt Herbs
Effect of different levels of Boswellia serrata Triana & Planch on immune responses of broilers chicks

Effect of different levels of Boswellia serrata Triana & Planch on immune responses of broilers chicks

Authors: Sayed Noradin Tabatabaei; Mehrdad Modaresi; Fariborz Moatar; Akbar Pirestani; Ehsan Tavalaeean
( 14 downloads)
Abstract

Introduction & Aim: The aim the present study is to examine the effects of Boswellia serrata Triana & Planch on (immune response) performance and carcass characteristic in broiler chicks. Experimental: In this study, 360 one day old chicks (Ross 308) were allocated to six treatments with four replicates in a completely randomized design for a six week period. Treatments consisted of the basal diet supplemented with 0.0 (control), 0.1, 0.15, 0.2, 0.3 and 0.5% B. serrata. Results & Discussion: The findings showed that feed intake of broilers fed control diet was significantly (p<0.05) higher than broilers fed 0.50% B. serrata in the grower period, also weight gain of broilers fed control diet was significantly (p<0.05) higher than broilers fed 0.30 and 0.50% B. serrata in the starter and grower period, but feed conversion ratio of broilers fed 0.50 % B. serrata was significantly higher than broilers fed control diet in the grower period. Application diet containing 0.15% of B. serrata led to the best feed conversion ratio in the total period, while the differences were not statistically significant. Percentage of gizzard to body weight, intestinal and seca length of broiler fed diet containing 0.5% of B. serrata was significantly (p<0.05) higher than broiler fed control diet.

Keywords: Effect of different levels of Boswellia serrata Triana, immune responses
Chemical components of essential oils of four Eucalyptus L'Her. species cultivated in Kashan’s botanical garden, Iran

Chemical components of essential oils of four Eucalyptus L'Her. species cultivated in Kashan’s botanical garden, Iran

Authors: Hossien Batooli; Abdolhamid Bamoniri; Abdolrasol Haghir Ebrahimabadi; Javad Safaei-Ghomi
( 15 downloads)
Abstract

Background & Aim: Eucalyptus L'Her. [an aromatic genera of the family (Myrtaceae)] is native to Australia and other tropical species is planted worldwide. The purpose of this study is to extract and identify the essential oils components of four species of Eucalyptus L'Her. cultivated in botanical garden of Kashan, Central Iran. Experimental: Leaves of E. largiflorens, E. intertexta, E. torquata and E. loxophleba, were collected from the botanical garden in Kashan. Extracting was done by hydro-distillation method and Clevenger approach. Components of essential oils were identified using GC and GC/MS analysis. Results & Discussion: The essential oil yield of E. largiflorens was 1.85% (v/w) and, nineteen compounds were identified. The major components in the essential oil were 1,8-cineol (58.71%), p-cymene (12.42%), α–pinene (5.61%) and terpinen-4-ol (4.53%). Yield of the essential oil of E. intertexta was 1.5% (v/w) and, sixteen components were identified which the major components were 1,8-cineol (69.45%), α–pinene (14.26%), globulol (4.02%) and terpinen-4-ol (1.79%). Yield of the essential oil of E. torquata was 1.2% (v/w) and, twenty components were identified which the main compounds were 1,8-cineol (33.86%), α–pinene (26.14%), aromadendrene (8.99%) and globulol (7.28%). The essential oil yield of E. loxophleba was 1.54% (v/w) which the major components were identified including 1,8-cineol (25.71%), globulol (21.63%), aromadendrene (20.43%) and 4-metyl-2-pentyl acetate (14/98%). The highest amount of terpenoides in essential oil of leaves E. largiflorens, E. intertexta, and E. torquata were monoterpenes, while the highest amount of terpenoides in essential oil of E. loxophleba were sesquiterpenes. Industrial and practical recommendations: According to the results of this research and the value of different species of medicinal Eucalyptus L'Her essential oils (in particular, the combination of 1,8-cineol), cultivation and exploitation of E. intertexta for pharmaceutical industry is recommended.

Keywords: Eucalyptus L'Her. 1; 8-cineol Essential oil Medicinal plants α–pinene
Chemical compositions, antibacterial and antioxidant properties of Echinophora cinerea essential oil

Chemical compositions, antibacterial and antioxidant properties of Echinophora cinerea essential oil

Authors: Maryam Pass; Marzieh Rashidipour; Gholamreza Talei; Behrouz Doosty
( 16 downloads)
Abstract

Background & Aim: Echinophora cinerea is a plant of the Apiaceae family, which reaches to a height of 30 to 100 cm. The essential oil of Echinophora cinerea contains alkaloids and flavonoids etc. Phenolic composition is one of the best sources of natural antioxidants. The goal of this study is to examine the antibacterial and antioxidant activities of the essential oil of E. cinerea. Experimental: In order preparation and identification of chemical compounds of essential oils, hydrodistillation approach and gas chromatography coupling with a mass spectrometer (GC, GC-MS) were used. Antioxidant capacity of the essential oil was determined by DPPH assay and, compared with standard antioxidants (BHT). Antibacterial test had done by broth microdilution and disk diffusion methods, and minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of these oils after 24 and 48 h were determined and compared to antibiotic standards against food-borne pathogens including Staphylococcus aureus, Listeria monocytogenes, Pseudomonas aeruginosa, Escherichia coli and MRSA. Results & discussion: Results of GC and GC-MS showed that the major components of essential oil of E. cinerea were α-phellandrene (32.09%), limonene (16.28%), p-cymene (10.75%), α-pinene (9.79%), carvacrol (3.79%) and β-myrcene (2.65%). Results of antioxidant test indicated that IC50 for oil of Echinophora cinerea was 740 μg/ml while for BHT was 50.6 μg/ml. The highest antibacterial effects of essential oil were observed on standard bacteria of Staphylococcus aureus with MIC= 0.16 μg/ml and MBC= 0.63 μg/ml. This effect on MRSA-resistant Staphylococcus and E. coli was weaker. Pseudomonas aeruginosa was resistant to essential oil. Industrial and practical recommendations: According to results of present study, the essential oil E. cinerea can be used in the food, pharmaceutical, cosmetic and health industry and flavoring.

Keywords: Echinophora cinerea Antioxidant Antibacterial GC-MS DPPH
Effect of foliar application of Jasmonic Acid (JA) on essential oil yield and its compositions of Thymus daenensis Celak

Effect of foliar application of Jasmonic Acid (JA) on essential oil yield and its compositions of Thymus daenensis Celak

Authors: Mohammad Ashrafi; Abdollah Ghasemi Pirbalouti; Mehdi Rahimmalek; Behzad Hamedi
( 15 downloads)
Abstract

Background & aim: Recent researches have been done on effects of plant growth regulators (PGR) on growth and production of different crops whose results indicate the effectiveness of most of these hormones on qualitative and quantitative growth of herbs. The application of regulators and inhibitors of growth on herbs may, in addition to growth, also lead to secondary metabolites stimulation. Jasmonic acid (JA) and its volatile methyl ester (MJ) collectively termed jasmonates, are regarded as endogenous regulators that play important roles in regulating stress responses, plant growth and development. This study was carried out to survey the effect of spraying of jasmonic acid (JA) on T. daenensis Celak in Shahrekord, Southwest Iran at 2010-2011. Experimental: A pot experiment was conducted in a CRD with six treatments and seven replications. The treatments including I: distilled water (control), II: distilled water and solution (acetone), III-VI: JA in various concentrations (50, 100, 200 and 400 µM). The treatments were applied before flowering stage. The aerial parts were characterized by Clevenger approach and Gas Chromatography–Mass Spectrometry. Results & discussion: Results of analysis of variance of the experiment showed that different levels of the foliar application of JA do have significant impacts on chemical constitutes in the essential oil of T. daenensis. Various concentrations of foliar application of JA caused decrease or increase some of secondary metabolites in essential oil. In comparison with other treatments, there is a significant difference between amount of thymol and carvacrol, as two main compounds in essential oil of T. daenensis, having 100 µM JA. The application of acetone solvent with distilled water also caused the increase in some of constitutes of the essential oil. Industrial and practical recommendations: In metabolic cultivation, for producing a special metabolite, the main agronomic and processing management should be regarded in order to increase the rate of essence and metabolites. Finally, some of secondary metabolite production in Thymus daenensis Celak could be partially changed by supplementation of different elicitors such as JA.

Keywords: Thymus daenensis Jasmonic acid Essential oil GC-MS Thymol Carvacrol
Effect of sowing season on growth and oil chemical composition of ajowain (Carum capticom L.) under salinity stress

Effect of sowing season on growth and oil chemical composition of ajowain (Carum capticom L.) under salinity stress

Authors: Soheila Dokhani; Forogh Mortezaei-Nejhad; Saied Davazdah-Emami
( 15 downloads)
Abstract

Background & aim: Saline lands can be utilized by planting native medicinal plants tolerant to salinity. The quantity and quality these plants are changed during various seasons in agriculture systems. Thus, this study carried out under salinity stress in order to examine the effect of sowing in two seasons on growth characteristics and essential oil components of ajowan (Carum capticum L.), Experimental: Different salinity treatments (EC= 0.3, 4, 8 and 12 dS/m) were used before flowering stage of ajowan in pot experimental at Khorasgan (Isfahan), Iran. Growth characteristics were measured and, the essential oil analyzed by GC/MS apparatus. Results & discussion: Results showed that increasing salinity caused decrease plant height in spring planting and increase in number of branching in summer planting. The amounts of plant height, number of umbel, number of flower in main umbel and number of floret in main umbel in summer sowing was higher than spring sowing. Interaction effects of salinity and sowing season on growth characteristics showed that the highest plant height was obtained from summer sowing under salinity 4 dS/m treatment and the lowest amount of plant height obtained from spring sowing under salinity 12 dS/m treatment. In spring and summer sowing, ten and thirteen components were identified in the essential oil of C. capticum, respectively. The major compounds in the essential oil of C. capticum were of thymol and γ-terpinene. Industrial and practical recommendations: According to results of this study, It seems that sowing of C. capticum under saline soils (~EC=12 dS/m) for metabolic agronomy (especially thymol) in spring sowing could be is suitable.

Keywords: Carum capticum L Salinity stress Essential oil GC-MS
Effect of jasmonic (JA) and salicylic acid (SA) on phytochemical properties of Salvia officinalis L.

Effect of jasmonic (JA) and salicylic acid (SA) on phytochemical properties of Salvia officinalis L.

Authors: Mehdi Rahimmalek; Shekoofe Azad; Mehrab Yadegari; Abdollah Ghasemi Pirbalouti
( 13 downloads)
Abstract

Background & aim: Sage (Salvia officinalis L.) is a perennial medicinal herb which belongs to Lamiales order and Lamiaceae family. This plant has economical value for four years. Nowadays the use of plant growth regulators (PGR) has an important role for increasing the secondary metabolites in plants. Among PGRs Jasmonic (JA) and salicylic acid (SA) have many applications in medicinal plants. Experimental: This study was carried out to survey the effect of spraying of various concentrations JA and SA on S. officinalis L. in field experiment of Hormozgan, South Iran at 2010-2011. A pot experiment was conducted in a CRD with ten treatments with three replicates. The treatments including I: distilled water (control), II: distilled water and solution (acetone), III-VI: SA in different concentrations (1, 10, 20 and 40 mol/l, VII-X: JA in various concentrations including 50, 100, 200 and 400 µM. The leaves were harvested at before flowering and, the essential oil extracted by Clevenger approach then the compounds were characterized using Gas Chromatography–Mass Spectrometry (GC-MS). Results & Discussion: Results showed that various concentrations of foliar application of JA and SA lead to decrease or increasing of some secondary metabolites in essential oil of S. officinalis L. Compounds such as a-pinene, camphene, b-pinene, 1,8-cineole, cis-thujane, trans-thujane, borneol, bornyl acetate and camphor were affected from JA and SA treatments. Industrial and practical recommendations: According to the results of present study it suggests to increase the major compounds of essential oil of Salvia officinalis L.using SA and JA.

Keywords: Salvia officinalis L. Jasmonic acid Essential oil GC-MS Salicylic acid
Effect of different treatments of organic fertilizers on growth characteristics and yield of Cassia angustifolia Vahl.

Effect of different treatments of organic fertilizers on growth characteristics and yield of Cassia angustifolia Vahl.

Authors: Amin Nickhah; Hassanali Naghdi Badi; Mohammad Hasan Shirzadi; Ali Mehrafarin; Niloufar Taherian
( 14 downloads)
Abstract

Background & aim: In sustainable agriculture, organic fertilizers are considered as important alternatives for chemical ones and are of great importance in organic producing of medicinal plants. This study was conducted to study the effects of organic fertilizers on Cassia angustifolia Vahl in dry and subtropical climate. Experimental: This study was carried out in Jiroft region, Kerman (Iran), at 2009-2010 in a randomized complete block design with three replications and thirteen fertilizer treatments. Results & discussion: The results of analysis of variance showed that treatments had significant effects on plant height, leaf width, number of branches, fresh weight of leaves, dry weight of leaves, fresh weight of shoot and dry weight of shoot (ppIndustrial and practical recommendations: In environmental conditions of the studied region, the integrated application of organic fertilizers containing nitrogen can improve quantitative yield of Cassia angustifolia Vahl. Therefore application of organic fertilizers can improve sustainable agriculture and organic production of medicinal plants in addition to decrease of chemical fertilizers application.

Keywords: Cassia angustifolia Vahl Organic fertilizer Dry climate Medicinal herbs
The effect of Policosanol on decrease of cholesterol, triglyceride and the other lipid metabolite levels in blood of Wistar rats

The effect of Policosanol on decrease of cholesterol, triglyceride and the other lipid metabolite levels in blood of Wistar rats

Authors: Fatemeh Foroohi; Mansor Amraei; Hadi Zamani
( 15 downloads)
Abstract

Background & Aim: Policosanol is a component from main alcohol groups with long-chain that extracted from cane sugar. In the present research, the effect of policosanol was investigated on decrease of lipid metabolite levels in blood of hypercholesterolemia rats. Experimental: A number of 32 male Wistar rats race with average weight of 250 g was divided into four groups including negative control group (n=8) that had normal diet, positive control and experimental groups I and II (n=8) with high cholesterol diet of 2% cholestroled, and respectively they received placebo and policosanol with concentrations 0.25 and 0.5 mg/day. After four weeks, following the treatment of animals and bloodletting, the biochemical traits such as cholesterol biochemical, triglyceride, and LDL-C and HDL-C factors were measured. Results & Discussion: The results showed that in experimental group (I) there was a significant decrease (p<0.05) in the rate of cholesterol, LDL-C and triglyceride, and significant increase (p<0.05) in HDL-C compared with control group. In experimental group II, there also was a significant decrease (p<0.01) in the rate of cholesterol, LDL-C and triglyceride, and significant increase (p<0.01) in HDL-C compared with control group. Industrial and practical recommendations: Policosanol can be an effective component in decreasing the rate of cholesterol, LDL-C and triglyceride and increasing HDL-C.

Keywords: Policosanal LDL-C HDL-C Cholesterol Triglyceride
Ethno-botany of medicinal plants by Mobarakeh’s people (Isfahan)

Ethno-botany of medicinal plants by Mobarakeh’s people (Isfahan)

Authors: Shaahin Mardani-Nejhad; Mansoureh Vazirpour
( 15 downloads)
Abstract

Background and aim: From long time ago, traditional medicine was integrated with popular culture and various aspects of treatment have been allocated to it. At the present time, the use rate of this method depends on people’s belief and recognition. Experimental: The research was conducted in the region Mobarakeh, Iran, in order to identify the priorities for application of medicinal plants by indigenous people. Results & Discussion: The results showed the more use of plants is in the field of gastrointestinal problems with frequency 17% and 53 used plant species. About 20 plant species were used for relieving diarrhea by indigenous people. Application frequency of effective plants on skin and hair was about 14% which eight plant species from 32 species were used for removing facial burble. Frequency of use as anti-inflammatory and anti-infection was about 11 % with 12 plant species. Frequency of effective application on circulatory system was 9% (eight medicinal plants). Frequency of use as urinary tract and kidneys was about 8% with 12 herb species. Effective applications of plants on endocrine problems, neurological problems and respiratory problems were seven, six and five percent. Medicinal plants also the effect on oral, dental and muscle problems respectively were three and two percent respectively. Industrial and practical recommendations: Examinations showed that most use of medicinal plants in the area is to remove ​​digestive problems. Also, awareness of these applications can be a policy for obtaining the new drug applications of these plants which has never been reported in available sources.

Keywords: Medicinal plants Traditional medicine Ethnobotany
Effect of explants and growth regulators on direct organogenesis of Dracocephalum kotschyi Boiss. via tissue culture technique

Effect of explants and growth regulators on direct organogenesis of Dracocephalum kotschyi Boiss. via tissue culture technique

Authors: Mahmoud Otroshy; Kosar Moradi
( 15 downloads)
Abstract

Background & Aim: Dracocephalum genus belongs to the Lamiaceae family which has eight species in Iran. Dracocephalum kotschyi Boiss is an important medicinal and aromatic plant that use in pharmaceutical industry. In addition, this species is used in Iranian traditional medicine. The aim of current study was to examine the various growth regulations and explants on direct organogenesis of Dracocephalum kotschyi using tissue culture technique. Experimental: Various concentrations of BAP, IBA and NAA belong to auxin and cytokinin, as two important plant growth regulators (PGR), with shoot tip explants; hypocotyl and cotyledon were investigated in plant micropropagation of Dracocephalum kotschyi. Results & Discussion: Results indicated that the best treatment and explants on regeneration Dracocephalum kotschyi was MS medium containing 5 mg/L BAP, and 0.2 mg/L NAA on shoot tip explants. Regenerated explants on MS medium containing 1 mg/L BAP and 0.5 mg/L IBA had elongation growth and the elongation growth medium, plantlets were rooted. Rooted plantlets were then transferred to the hardening. After consistency of the regeneration plant seedlings Dracocephalum kotschyi, about 95% of seedling transferred to greenhouse suitable growing. Industrial and practical recommendations: According to the results of this research, we can start to proliferate and produce the effective matter of medicinal plant, such as Dracocephalum kotschyi. The most important benefit of this method is to prevent from extinction and to protect the species out of the environment.

Keywords: Dracocephalum kotschyi Explants Plant growth regulators Organoganasis
Effect of different distillation methods on quantity and quality of essential oil of two Ziziphora L. species

Effect of different distillation methods on quantity and quality of essential oil of two Ziziphora L. species

Authors: Hosayn Batooli; Maryam Akhbari; Seyed Mohammad Javad Hosseinizadeh
( 13 downloads)
Abstract

Background & Aim: Ziziphora L. genus belongs to the Lamiaceae family, which has important medicinal and aromatic plant species. In current study, essential oil chemical compositions of two Ziziphora L. species (Z. tenuior L. and Z. clinopodioides Lam.) were investigated. Experimental: The flowering branches of Z. tenuior and Z. clinopodioides were collected in spring and summer 2011. The aerial parts dried in shade at room temperature. The flowering branches of these species subjected to volatile fraction were isolated by simultaneous distillation extraction (SDE), hydro distillation (using a Clevenger-type apparatus), steam-cooled distillation (ultrasonic) and steam distillation. The analysis of the oils was performed by using GC and GC-MS. Results & Discussion: The results indicated the essential oil yield of Z. tenuior ranged 0.2% to 2.3% (w/w), and Z. clinopodioides ranged 0.3% to 1.3% (w/w(. In total, 18 components were identified in Z. tenuior essential oil, and 33 components were identified in Z. clinopodioides essential oil. The main components of Z. tenuior essential oil were pulegon (80.01% - 90.10%), and piperitenone (4.5% - 7.14%). The major compounds in Z. clinopodioides essential oil were pulegon (25.87% - 35.20%), piperitenone (10.11% - 27.88%), menthol (11.41%-17.50%), and menthon (0 - 7.69%). The highest percentage of menthol in essential oil in two species obtained from SDE method. Industrial and practical recommendations: According to the purity 81% of pulegon combination in essential oil of Ziziphora with 2% essential oil yield, can be purified in production of Ziziphora.

Keywords: Ziziphora Essential oil Lamiaceae Pulegon Aromatic Plants
Ethnobotanical study of medicinal plants in Natanz region (Kashan), Iran

Ethnobotanical study of medicinal plants in Natanz region (Kashan), Iran

Authors: Shabnam Abbasi; Saied Afsharzadeh; Abdolreza Mohajeri
( 16 downloads)
Abstract

Background & Aim: Iran has a long history in ethnobotany and ethnopharmacology. The indigenous people in YahyaAbad area, is located in the conserved area of the Karkas mountains, are used many plants and natural material for treatment their diseases. YahyaAbad area is an important part of central mountains of Kashan, Central Iran. There are many plant species in this area which have medicinal uses. The aim of this research was ethnobotanical study on medicinal herbs of YahyaAbad area in order to recognize custom, tradition, and application way of plants by the people. Experimental: In current study, plant species were collected at several times. Plant identities were confirmed by botanist and references. An analysis was made of the species used, parts of the plant employed, preparation methods, administration means, and the ailments treated in relation to pathological groups. Results & Discussion: In total, 65 medicinal plant species collected and identified in YahyaAbad area. Results indicated some of medicinal plants are used commonly by the indigenous people. Many of medicinal plants are used for eliminating different pains. Generally, some of the uses were found to be new when compared with published literature on ethnomedicine of Iran. Industrial and practical recommendations: According to medicinal value of some plants in studied area, and non suitable climate condition such as droughts which caused to intensify livestock's grazing and irregular plants harvest, it seems using adopting the appropriate methods and conservation policies, we can prevent from this issue.

Keywords: Ethnobotany Medicinal plants The Karkas mountain
Essential fatty acids extracted from Portulaca oleracea L. leaves

Essential fatty acids extracted from Portulaca oleracea L. leaves

Authors: Jila Asghari; Sohreh Alimohammadzadeh; Mohsen Mazaheritehrani
( 15 downloads)
Abstract

Background & Aim: Purslane (Portulaca oleracea L.), belongs to the Portulacaceace family, grows in many parts of Iran. Results of previous studies were indicated purslane is a rich source of essential fatty acids of omega-3 and omega-6. Portulaca oleracea is a widespread weed, being the eighth common plants of the world. The Aim of current study was to compare the essential fatty acids composition in two populations of Portulaca oleracea from Gorgan and Sardasht regions. Experimental: The oil of purslane leaves was extracted by Soxhlent apparatus using petroleum ether. Extracted oil was change into methyl ester, before analysis by gas chromatography. Results & Discussion: The total fat contents of Gorgan and Sardasht populations were 4.71% and 4.81%, respectively. In both populations, three the essential fatty acids were detected. Linolenic acid, ranged from 105.43 to 148.97 mg FA/g, linoleic acid ranged from 30.13 to 39.31 mg FA/g, and the lowest was recorded for Arashidonic acid which ranged from 5.16 to 8.30 mg FA/g for the Gorgan and Sardasht populations, respectively. Industrial and practical recommendations: The oil and the essential fatty acids content of purslane leaves in the Sardasht population were higher than the Gorgan population. Since the oil and essential fatty acid content were affected by different climatic and cultivation factors, the western province of Iran is better suited for cultivation of medicinal plants having great oil and essential fatty acid contents.

Keywords: Portulaca oleracea L Essential fatty acids Linolenic acid Linoleic acid
Cytogenetic study of two Alhagi species

Cytogenetic study of two Alhagi species

Authors: Maryam Ebrahimpour Norabadi; Zahra Yazdanbakhsh; Maryam Keshavarzi
( 15 downloads)
Abstract

Background & Aim: Alhagi genus has two important medicinal plant species in Iran, including Alhagi pseudoalhagi, and A. graecorum (known “Kharshotor” in Persian). These plants are distributed in Iran like weeds. Generally, Alhagi is used as an anti-hemorrhoid, laxative and diuretic. Manna in both species produced by activity of a kind of insect on Alhagi branches which has medicinal properties and been used in Iranian traditional medicine. In this study, cytogenetic of 17 populations of A. graecorum and A. pseudoalhagi were studied. Experimental: In order to study the cytogenetic, pollen mother cells were studied in different meiotic developmental steps, and photographed. Pollen fertility test was done for studied populations. Data were statistically analyzed by SPSS software. Results & Discussion: Chromosome number in all species were 2n=2x= 16. Studied populations showed regular meiosis while some abnormalities as anaphase bridge in anaphase I, chromosomes stickiness in metaphase I, laggard chromosomes in metaphase I, and anaphase I, presence of rod and circular bivalent, univalent, quadrivalent and tri-polar were observed. Such structural modifications cause to increase in genetic variation and adaptation to environmental conditions. B chromosomes (I-V) were observed. Industrial and practical recommendations: Results of current study can lead to further researches in order to increase and optimize the medicinal effective substances in Alhagi by genetic modification.

Keywords: Alhagi Cytogenetics Meiotic behavior Abnormalities

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