Journal of Advanced Biotechnology and Experimental Therapeutics

Journal of Advanced Biotechnology and Experimental Therapeutics

Basic info

  • Publisher: Bangladesh Society for Microbiology, Immunology, and Advanced Biotechnology
  • Country of publisher: bangladesh
  • Date added to EuroPub: 2019/Feb/20

Subject and more

  • LCC Subject Category: Biological Sciences, Genetics, Microbiology, Pharmacology, Toxicology
  • Publisher's keywords: Cellular and Molecular Biology, Genetics and Genomics, Immunology and Physiology, Metabolism and Diseases, Microbiology and Infection, Pharmacology and Toxicology, Plant Biology and Herbal Medicine, Stem Cells and Cancer Biology, Reproductive and Aquatic Biology
  • Language of fulltext: english
  • Full-text formats available: PDF, HTML

Publication charges

  • Article Processing Charges (APCs): Yes 30 USD
  • Submission charges: No
  • Waiver policy for charges? No

Editorial information

Open access & licensing

  • Type of License: CC BY-NC
  • License terms
  • Open Access Statement: Yes
  • Year open access content began: 2018
  • Does the author retain unrestricted copyright? True
  • Does the author retain publishing rights? True

Best practice polices

  • Permanent article identifier: DOI
  • Content digitally archived in: Nopolicy
  • Deposit policy registered in: None

This journal has '24' articles

Analysis of telomere length in the coronary artery to determine the pathogenesis of coronary artery disease

Analysis of telomere length in the coronary artery to determine the pathogenesis of coronary artery disease

Authors: Mahaboob V Shaik, Subrahmanyam G
( 0 downloads)
Abstract

Endothelial dysfunction is one of the earliest pathological features in atherosclerosis. The current study designed to analyse the telomere length of human coronary artery derived endothelial cells to identify the relationship between telomere shortening and coronary artery disease (CAD)s. Coronary artery endothelial cells obtained from 30 patients with CAD and cell line were analysed by real time PCR method. Relative telomere and single-copy 36B4 gene (T/S) ratio which corresponds to the telomere length in coronary artery was significantly smaller in coronary artery disease patients than compared with control cell line. Flow cytometry analysis resulted endothelial cell count ranged from 0.55 x 106 to 0.96 x 106. Findings of this study suggest that telomere shortening in coronary artery endothelial cells play important role in the coronary artery disease.

Keywords: Coronary artery disease, telomere length, endothelial dysfunction
Prophylactic effects of vitamin E and coriander (Coriandrum sativum) extract on lead-induced testicular damage in Swiss albino mice

Prophylactic effects of vitamin E and coriander (Coriandrum sativum) extract on lead-induced testicular damage in Swiss albino mice

Authors: Mohammad Rafiqul Islam, Md. Anwar Jahid, Md Zahirul Islam Khan and Md. Nabiul Islam
( 0 downloads)
Abstract

Lead toxicity is the vital issue in the developing countries that causes serious health hazards in animals and humans. Prophylactic effects of vitamin E and coriander (Coriandrum sativum) extract on lead-induced testicular damage in Swiss albino mice were investigated in the present study by gross and histological studies in five groups (control, lead intoxicated, vitamin E-treated, coriander-treated, vitamin E and coriander-treated). Treatment was done for 42 days. In the present study, weight and length of left and right testes were significantly (p<0.05) reduced in lead-intoxicated mice in comparison to the control group. Lead acetate was found to cause separation of primary spermatocyte from spermatogonia, morphological changes of seminiferous tubule and irregular arrangement of spermatogenic cells in the seminiferous tubules of testis. Vitamin E and coriander extract were found effective in the treatment of lead intoxicated mice and testes histology were found normal. Combined antioxidative action of vitamin E and corriander extract was also found effective in the treatment of lead intoxicated mice. The present investigation may serve as baseline data about adverse effects of lead toxicity and efficacy of vitamin E and coriander extract against lead toxicity. Further research need to be done to isolate and purify the active principle involved in the antioxidant activity of this plant.

Keywords: Vitamin E, coriander, testis, mice, lead, toxicity
A brief review on Japanese Encephalitis in Swine with its diagnostic strategies

A brief review on Japanese Encephalitis in Swine with its diagnostic strategies

Authors: Madhusmita Ghana and Pratyush Kumar Das
( 0 downloads)
Abstract

Japanese encephalitis is a viral disease affecting humans, mostly the children and is a major concern to public health. The single stranded flavivirus is mainly transmitted by mosquitoes of the Culex genus and causes neurological disorders with high fatality. The virus is well amplified in animals like pigs (swine) that act as a host. Close proximity of the domesticated pigs to human settlements increases the risk of human infection. The animal to human zoonoses of this antigen is occured through the mosquitoes bite. Recent developments have led to discovery of various vaccines for the treatment of the disease. This review discusses various aspects of the Japanese encephalitis disease with their transmission, pathogenesis and diagnostic strategies. Moreover it highlights the importance of prevention of transmission of the disease over its cure by proposing a hypothetical kit design model to detect presence of the Japanese encephalitis antigen in the swine populations. This detection process may help to detect infected animals and thus help to keep such animals away from the human settlements so as to prevent transmission as well as outbreak of the disease. This may further help to create a disease free environment, thus saving many lives.

Keywords: Japanese encephalitis, Flavivirus, Vector, Gold nanoparticles, Swine, Kit design
A reliable and easy method to isolate a pure population of bovine granulosa cells from slaughterhouse ovaries for in vitro studies

A reliable and easy method to isolate a pure population of bovine granulosa cells from slaughterhouse ovaries for in vitro studies

Authors: Md Mahmodul Hasan Sohel
( 0 downloads)
Abstract

In a growing follicle, granulosa cells (GCs) are the major cellular component and perform several important functions. GCs are an excellent choice for in vitro experimentation to understand their functions in vivo. However, the isolation of a pure fraction of GCs from slaughterhouse ovaries remains a challenge. The current study describes a simpler yet reliable method for isolating GCs from slaughterhouse ovaries of domestic animals. For this, bovine ovaries were collected from a nearby slaughterhouse and GCs were collected by aspiration method, one fraction of fresh GCs was snap frozen and stored at -80°C for further analysis and another fraction was used in culture purpose. The purity of GCs was determined by quantifying follicle stimulating hormone receptor (FSHR) and CYP17A1 transcripts and analyzing the cultured cells under a microscope. The viability of GCs ranged from 66 – 35 % depending on the time and media used in transportation-processing. The results showed that FSHR gene was highly expressed as evidenced by the presence of a strong band while CYP17A1 is completely absent in the GCs isolated using the current method. In addition, the characteristics of the cultured cells further confirm the purity of isolated GCs. The current method isolates a pure population of GCs from slaughterhouse ovary without any contamination of thecal cells.

Keywords: Ovary, Granulosa cells, Isolation method, Bovine, Gene expression
Spectrochemical characterization of Vero cell line against PPR virus infection

Spectrochemical characterization of Vero cell line against PPR virus infection

Authors: Md. Mizanur Rahman, Kumar Jyotirmoy Roy, Mst. Khudishta Aktar, Md. Rafiqul Islam, Md. Abdul Kafi
( 0 downloads)
Abstract

An in vitro virus infectivity assay based on the newly introduced spectrochemical method was performed using Peste des Petits Ruminants (PPR) virus infected Vero cell line as a model infection. Herein, the mitochondrial reductase enzyme activity was monitored using 3-(4,5-Dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) reagent as a substrate which was reduced to a insoluble formazan products. The purple to darker color was achieved upon hydrolysis of the insoluble formazan with DMSO solution. The absorbance (OD values) of colored solution was measured by spectrophotometer at a wave length of 570nm. The infectivity/viability data were achieved from the OD values of different doses of virus infected or non-infected cells using the standard formulae. The OD values obtained from spectrochemical assay were compared with traditional Plaque assay and validated with Trypan blue assay. The data obtained from spectrochemical analysis showed similar trend as was achieved with traditional methods with a little variation in the sensitivity. The sensitivity variations are obvious due to the marked differences in the measurement unit and detection methods. However the newly introduced spectrochemical method showed superiority over the traditional methods because of its simple, label free, less time consuming measurement method and its suitability in the monitoring of large number samples.

Keywords: Spectrochemical-Assay, PPR virus, MTT assay, Plaque assay, Trypan blue assay
Insilico analysis of γ- aminobutyric acid transaminase (GABA-T) of Brassica napus (Rape)

Insilico analysis of γ- aminobutyric acid transaminase (GABA-T) of Brassica napus (Rape)

Authors: Shahidur Rahman and Md. Shahjahan
( 0 downloads)
Abstract

γ- aminobutyric acid (GABA) is an essential metabolite which plays a crucial role in signal transmission, stress metabolism, and some other activities also reported. Although the actual function of GABA shunt is not clearly understood. Three key enzymes, gamma aminobutyric acid transaminase (GABA-T), succinic semialdehyde dehydrogenase and succinic semialdehyde reductase are involved in GABA shunt mechanism en route from glutamate to the tricarboxylic acid cycle (TCA)which could pave the way of GABA shunt action. The enzyme gamma aminobutyric acid transaminase (GABA-T) could also play a key role in GABA shunt action by converting GABA to succinic semialdehyde (SSA).In this study, the protein sequence of γ-aminobutyric acid transaminase of Brassica napus (Rape) was retrieved from UniProt protein database and analyzed GABA-T enzyme using different bioinformatics tools and servers to analyze the physiochemical properties, amino acid composition, conformational states, and 3D structure. We found that our experimental protein sequence was very unstable, and the graph of Local Quality Estimate showed that the sequence was porn to mutation and value of Z score was above two in comparison with a non-redundant set of PDB structure. In addition, the phylogenetic tree revealed that GABA-T of Dendrobium officinale, Phoenix dactylifera, Oryza sativa, Arabidopsis thaliana and Brassica napus evolved from a common ancestor gene.

Keywords: GABA-T, TCA cycle, UniPort, metabolism, transmission, stress
The possible regulations through cross-generation transmission on childhood obesity

The possible regulations through cross-generation transmission on childhood obesity

Authors: Vo Truong Nhu Ngoc and Dinh-Toi Chu
( 0 downloads)
Abstract

Obesity and overweight are considered as one of the top health problem worldwide, obesity may induce both physical and mental health consequences [1, 2]. Obesity and overweight is regulated by both genetics and environmental factors which can control the balance between the lipid accumulation and energy expenditure, thus several bio-functional markers [3] of adipose tissues involve in the regulation of obesity, and obesity is also regulated by developmental age and nutrition [4]. Two interesting findings in the flied of lipid metabolism and obesity have been recently released on Pediatric Obesity by R. Somerville et al [5] and on Nature Medicine by Wenfei Sun et al [6]. Both studies focused on a very important topic in childhood obesity that is “How do a life course approach and living environment of previous generations affect lipid metabolism and obesity in the child?”. The work done by R. Somerville et al [5] showed that the central adiposity in grandmother, not grandfather, was positively consistent with that in children at the age of 5 and 9. However, cold exposure (CE) in the father, not mother, before conception may benefit for systemic metabolism as well as prevent overweight and obesity in the children as proved in the study done by Wenfei Sun et al [6]. In both humans and animals, CE increases the browning of white adipocytes and the thermogenesis of both brite (the brown adipocytes induced in white fat depots) and classical brown adipocytes, these effects lead to reduction in lipid accumulation and body fat, but increase in glucose and insulin sensitivity as well as systemic metabolism [7-12]. As results, CE was proved to reduce overweight and diet-induced obesity [10, 13]. The first report was conducted in a prospective cross-generational cohort of 1094 children (5 and 9 years old), 1082 mothers, and 745 grandparents of these children [5]. As waist circumference (WC) in one of indicator for determining obesity and overweight [14, 15], authors have performed mediation analysis on WC of study cohort and found a significant positive relationship of grandmother WC and grandchildren WC, but they did not see that correlation in WC between grandfather and grandchildren. This result indicates that cross-generation transmission maybe one of factors regulating childhood obesity (Fig. 1A). The second report shows that environmental effects on father lead to changes in the systemic metabolism of offspring [6]. This has opened potential ways to control lipid metabolism and prevent obesity in human by optimizing ambient temperature at parents’ living places. It has been known that cold exposure activates the classical brown adipose tissue and induces functionally brite adipocytes in white fat depots in both human and animal models. The function of these thermogenic (brown and brite) adipocytes can improve the systemic metabolism and reduce fat mass [16-18, 12, 19], thus the cold exposure is considered as a potential therapy for controlling obesity and overweight [16, 18, 12]. In the current report [6], Wenfei Sun et al not only proved the anti-obesity effect of cold exposure but also went to a further step, because they showed that the browning effect of CE could transfer through from generations. Analyzing a cohort of 8,440 subjects, researchers found higher activities of thermogenic adipocytes in both brown (BAT) and white (WAT) fat tissues in children from the parents who were exposed to cold before impregnation or during pregnancy. Further investigations by authors showed that the CE effects on brown/brite adipocytes were only regulated through the paternal lineage. These findings in humans were supported by the studies in mice, they found that higher thermogenesis and respiration were partially induced by an increase in BAT activation of pups from the fathers exposed to the cold, and the paternal CE (P-CE) could improve systemic metabolism and protected mouse offspring from diet induced obesity. Mechanically, investigators proved that the increase in BAT function of offspring maybe due to the changes in brown adipogenesis and neurogenesis induced by P-CE, and the elevated formation of thermogenic cells in offspring stimulated by P-CE was a cell autonomous manner. Thus, the important findings by Wenfei Sun et al [6] suggest that CE in the father before conception may benefit for systemic metabolism as well as prevent overweight and obesity in the child (Fig. 1B).

Keywords: possible regulations, cross-generation, transmission, childhood obesity
Herbal contraceptive effect of Abrus precatorius, Ricinus communis, and Syzygium aromaticum on anatomy of the testis of male Swiss albino mice

Herbal contraceptive effect of Abrus precatorius, Ricinus communis, and Syzygium aromaticum on anatomy of the testis of male Swiss albino mice

Authors: Sonali Bhakta, Md Abdul Awal, Shonkor Kumar Das
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Abstract

The present research was designed to investigate in vivo herbal contraceptive effects on the anatomy of the testis in male Swiss albino mice (Mus musculus) and to observe that whether the herbal extracts depart any baleful effects on the genitalia or not. Swiss albino mice at age of 30 days were divided into two groups: control and treated group. From day 60, the treated group was administered orally with an aqueous extract (4.4 mg/kg body weight) prepared from the herbal plants. At day 97 (after treatment for 6 weeks) both the control and treated mice were sacrificed. Testes were collected for anatomical (gross and histomorphological) studies. Hematoxylin and Eosin staining was performed for the histo-morphological studies. In the present study, the gross anatomy of the testis were significantly (p<0.05) reduced and pale in color in treated mice in comparison to the control group. Histologically, number of seminiferous tubules, sertoli & leydig cells and amount of spermatozoa within the lumen of seminiferous tubules were decreased. Derangement of the seminiferous tubules along with the presence of a thick fibrous layer and vacuoles were found within and between the seminiferous tubules in the treated group. The aforesaid changes with a good contraception rate (75%) and devoid of any baleful effects even on the female were found, where the contraceptive pills are known to have many side effects. Considering above all, the mixture of plants seems to be effectively worked on the male gonad (testis) suggesting that the herbal extracts might be future use-worthy.

Keywords: Herbal contraception, anatomy, testis, Swiss albino mice
Prophylactic effects of vitamin E and coriander (Coriandrum sativum) extract on lead-induced testicular damage in Swiss albino mice.

Prophylactic effects of vitamin E and coriander (Coriandrum sativum) extract on lead-induced testicular damage in Swiss albino mice.

Authors: Mohammad Rafiqul Islam, Md. Anwar Jahid, Md Zahirul Islam Khan and Md. Nabiul Islam
( 0 downloads)
Abstract

Lead toxicity is the vital issue in the developing countries that causes serious health hazards in animals and humans. Prophylactic effects of vitamin E and coriander (Coriandrum sativum) extract on lead-induced testicular damage in Swiss albino mice were investigated in the present study by gross and histological studies in five groups (control, lead intoxicated, vitamin E-treated, coriander-treated, vitamin E and coriander-treated). Treatment was done for 42 days. In the present study, weight and length of left and right testes were significantly (p<0.05) reduced in lead-intoxicated mice in comparison to the control group. Lead acetate was found to cause separation of primary spermatocyte from spermatogonia, morphological changes of seminiferous tubule and irregular arrangement of spermatogenic cells in the seminiferous tubules of testis. Vitamin E and coriander extract were found effective in the treatment of lead intoxicated mice and testes histology were found normal. Combined antioxidative action of vitamin E and corriander extract was also found effective in the treatment of lead intoxicated mice. The present investigation may serve as baseline data about adverse effects of lead toxicity and efficacy of vitamin E and coriander extract against lead toxicity. Further research need to be done to isolate and purify the active principle involved in the antioxidant activity of this plant.

Keywords: Vitamin E, coriander, testis, mice, lead, toxicity

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