Detection of candidatus liberibacter asiaticus associated with citrus greening (huanglongbing) of mandarin by template preparation

Detection of candidatus liberibacter asiaticus associated with citrus greening (huanglongbing) of mandarin by template preparation

Journal

  • Journal title: Scientific Journal of Medical Science
  • ISSN: 2322-5025 (online)
  • Publisher: Sjournals
  • Country of publisher: iran, islamic republic of
  • Platform/Host/Aggregator: Sjournals
  • Date added to EuroPub: 2017/Feb/20

Subject and more

  • LCC Subject Category: Dentistry and Oral Surgery, Nursing, Pathology and Forensics, Pharmacology, Pharmacy, Physiology, Surgery, Toxicology
  • Publisher's keywords: Detection, PCR, Candidatus liberibacter, Asiaticus, Mandarin
  • Language of fulltext: english
  • Full-text formats available: PDF
  • Time From Submission to Publication: 3

AUTHORS

    G.P. Jagtap| Department of Plant Pathology, College of Agriculture, M.K.V., Parbhani – 431 402 (MS)., M.V. Mahajan| Department of Plant Pathology, College of Agriculture, M.K.V., Parbhani – 431 402 (MS)., D. Utpal*| Department of Plant Pathology, College of Agriculture, M.K.V., Parbhani – 431 402 (MS).

FULL TEXT

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ABSTRACT

The polymerase ChainReaction (PCR) diagnosis is more reliable and sensitive diagnostic tool forgreening bacterium than other conventional approaches like Electron microscopy,DNA-DNA hybridization and immunofluorescence (IF) for detection of citrusgreening. During experiment, it was observed that sodium sulphite method of DNAisolation provided higher yield and better quality DNA than other methods.Primer C (450 bp) was more efficient in amplifying the DNA of greeningbacterium even at a very low concentration of 0.1 pg. To confirm thereliability of PCR, the greening bacterium was also detected ingraft-inoculated plants, which showed typical greening bacterium was alsodetected in graft-inoculated plants, which showed typical greening symptoms.Results showed amplification of 450 bp in PCR suggesting sampling in March ismore suitable for PCR detection of greening bacterium.

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