• Journal title: Scientific Journal of Medical Science
• ISSN: 2322-5025 (online)
• Publisher: Sjournals
• Country of publisher: iran, islamic republic of
• Platform/Host/Aggregator: Sjournals
• Date added to EuroPub: 2017/Feb/20

• LCC Subject Category: Dentistry and Oral Surgery, Nursing, Pathology and Forensics, Pharmacology, Pharmacy, Physiology, Surgery, Toxicology
• Publisher's keywords: Detection, PCR, Candidatus liberibacter, Asiaticus, Mandarin
• Language of fulltext: english
• Full-text formats available: PDF
• Time From Submission to Publication: 3

G.P. Jagtap| Department of Plant Pathology, College of Agriculture, M.K.V., Parbhani – 431 402 (MS)., M.V. Mahajan| Department of Plant Pathology, College of Agriculture, M.K.V., Parbhani – 431 402 (MS)., D. Utpal*| Department of Plant Pathology, College of Agriculture, M.K.V., Parbhani – 431 402 (MS).

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The polymerase ChainReaction (PCR) diagnosis is more reliable and sensitive diagnostic tool forgreening bacterium than other conventional approaches like Electron microscopy,DNA-DNA hybridization and immunofluorescence (IF) for detection of citrusgreening. During experiment, it was observed that sodium sulphite method of DNAisolation provided higher yield and better quality DNA than other methods.Primer C (450 bp) was more efficient in amplifying the DNA of greeningbacterium even at a very low concentration of 0.1 pg. To confirm thereliability of PCR, the greening bacterium was also detected ingraft-inoculated plants, which showed typical greening bacterium was alsodetected in graft-inoculated plants, which showed typical greening symptoms.Results showed amplification of 450 bp in PCR suggesting sampling in March ismore suitable for PCR detection of greening bacterium.