The effect of some of the Iranian medicinal plants on Brucella Abortus on In-vitro and In-vivo
Journal Title: Journal of Herbal Drugs - Year 2010, Vol 1, Issue 1
Abstract
Introduction & Aim: The ethanol and aqueous extracts of Alhagi camelorum Fisch. (Fabaceae) whole plant, Scrophularia desertii Del. (Scrophulariaceae) whole plant and ethanol extracts of Echinophora platyloba DC. (Apiaceae) whole plant, Teucrium polium L. (Lamiaceae) whole plant and Berberis integerrima Bunge root brake were investigated for anti-Brucella in-vitro and in-vivo conditions. Experimental: Clinical isolated of Brucella abortus was studied using the disk diffusion method. Brucellosis was induced by repeated injection of Brucella abortus on Balb/C mice. In Balb/C model, we evaluated factors included primary weight/secondary weight ratio (W1/W2), spleen weight, spleen weight/body weight ratio and antibody titer with Wright method. Results & Discussion: The results of disk diffusion approach that the extracts from different plant species studied showed antibacterial activity against B. abortus, with the diameters of zone of inhibition ranging between 1 and 27 mm. Of the plants studied, the most active extracts at the concentration 500 µg/ml were those obtained from aqueous extracts of A. camelorum whole plant and showed strong inhibitory effects (zone of inhibition ≥15 mm) but ethanol extract of S. desertii at the concentration 125 µg/ml showed lowest of inhabitation effect. The bacteria in the study were sensitive to some of antibiotics, erythromycin and streptomycin being the most sensitive (inhibition zone values of 21 and 23 mm, respectively), while was resistant to some of the plant extracts at the concentration 62.5 µg/ml. The best results for W1/W2 were obtained from extract-treated by ethanol extract of B. integrrima root in dose of 400 mg/kg/day and following treated by erythromycin in dose of 400 mg/kg/day. The lowest of Spleen weight were obtained from aqueous extract of A. camelorum and following treated by erythromycin. The best results for Spleen W/Body were obtained from extract-treated by aqueous extract of A. camelorum and following treated by erythromycin in dose of 200 mg/kg/day. Also, the best results for antibody titer with Wright method were obtained from ethanol extract B. integrrima root brake. However, the results of mean of comparison with Duncan's test for antibody titer with Wright method showed no significant difference.
Authors and Affiliations
Abdollah Ghasemi Pirbalouti; Mohammad Reza Ghasemi; Hasan Momtaz; Ahmad Reza Golparvar; Behzad Hamedi; Lohraseb Shahgholian
Keywords
Related Articles
- EP ID EP23304
- DOI -
- Views 558
- Downloads 31
Effects of aluminosilicate zeolite application on morphological traits and chemical compositions of Aloe (Aloe vera L.) under drought stress
Background & Aim: Aloe vera is used in the food and pharmaceutical as an aid to effective treatment. The purpose of this study is to improve growth, increase production of valuable metabolites and increase the sugars in...
The methodology of research in Traditional Medicine
In countries having traditional medicine (TM), one of the main challenges for any researcher is research methodology. Current methodologies are different with what was in past. The main purpose of this article is to pres...
Effect of ecological factors on chemical compounds of Verbascum songaricum leaves
Background & Aim: Verbascum songaricum is a perennial herbaceous belongs to the Scrophulariaceae (snapdragon) family. Current study was done to study the environmental conditions effect on phytochemical properties of Ver...
Ethnobotanical study of Sojasrood medicinal plants (Zanjan Province)
Background & Aim: Nowadays, medicinal plants play a special role in the health and well-being of the community and they are always at the forefront of scientific and research centers. Traditional medicine and ethnobotany...
Chemical compositions of the essential oil of Gundelia tournefortii L. (Asteraceae) from Central Zagros, Iran
Background & Aim: Gundelia tournefortii L. (Tumbleweed) has been identified as one of the well-known and widely distributed plants with various applications in some of Asian and North African countries. It belongs to...