Evaluation of Hormone Treatment on Callus Length and Weight in Summer Safflower Cultivars
Journal Title: Universal Journal of Environmental Research and Technology - Year 2012, Vol 2, Issue 1
Abstract
With respect to country need to edible oils, it is more important to extend the cultivation of oilseeds. Although, safflower is the native plant of Iran and it is resistant to drought and salty stresses, but a few studies have been done on it. Today, improvement and manipulating of some of undesirable traits of safflower is possible by genetic engineering tools. During the manipulating process, important stages of tissue culture specially, regeneration phase of deformed plant has high worth. In order to evaluate the effect of explants, type of hormones and hormone levels on callus length and weight in summer safflower cultivars, an experiment was carried out in factorial based on randomized complete block design with 3 replications and four factors (safflower cultivars, explants, type of hormones and hormone levels ) in 2011. Results showed that safflower cultivars (Iraqi 222 and 34069) and explants (hypocotyle and cotyledon) had a significant effect on the callus length. Based on the results, the effect of hormones and hormone levels were significant on callus weight. 34069 and hypocotyle were known as a premier compounds due to maximum length of callus and Iraqi 222 at both explants were known as low yield compound due to the shortest callus length. Also it was revealed maximum callus weight was related to hormone levels (2 mg per liter) and Zeatin hormone.
Authors and Affiliations
Ali Reza Babazadeh Bedoostani, Behnam Tahmasebpour, Gader Ghaffari
Keywords
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