Analysis of genetic diversity in barley genotypes using storage proteins
Journal Title: International Journal of Farming and Allied Sciences - Year 2013, Vol 2, Issue 14
Abstract
Genetic variation is considered as a fundamental factor to plants breeding and makes it feasible to select plants with suitable traits. One of the methods to study genetic variation among plant species is using electrophoresis of seed storage proteins. In this research genetic diversity of 23 cultivars of barley genotypes with the use of Polyacrylamide gel electrophoresis SDS-PAGE was performed. Based on the revealing results, 21 bands (markers) in sheet of Polyacrylamide gel were apparent and the number of bands in each marker was different which indicates genetic diversity in genotypes under studies. According to tree diagram resulting from cluster analysis, these genotypes are in 3 groups. The amount of genetic diversity in genetic locus 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17and 18 respectively are equal to 0.49, 0.5, 0.09, 0.49, 0.38, 0.34, 0.34, 0.34, 0.28, 0.38, 0.38, 0.28, 0.5, 0.47, 0.38, 0.47 and 0.38 and its mean equals 0.37. According to the present results and high diversity in protein markers, these markers can be used to identify differences between varieties.
Authors and Affiliations
Ali Reza Shekhghasemi, Hossein Shahbazi, Ali Akbar Imani and Marefat Ghasemi
Keywords
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