Assessment Of Total RNA Extraction From Edible Mushroom (Agaricus bisporus) With Three Current Methods

Journal Title: International Journal of Farming and Allied Sciences - Year 2013, Vol 2, Issue 18

Abstract

Nucleic acid extraction is an attractive alternative to labor-intensive manual methods. In this report, a few methods of total RNA Extraction from Edible mushroom (Agaricus bisporus) compared. This methods consist AGPC(Acid Guanidinium-Phenol-Chloroform), The method according by Lithium chloride and RNx total RNA isolation Kit (CinnaGen). Best method selected for the preparation of high quality and intact total RNA from A. bisporus. Based on the Ethidium Bromide straining pattern of 28s and 18s rRNAs, either denaturing (formaldehyde/agarose) or nondenaturing (TAE/agarose) gel electrophoresis could be used to determine the general integrity of the RNA preparation. Analysis by either method revealed sharp rRNA bands indicative of high quality RNA. yield of RNA was acceptable in any three method but RNA quality in AGPC method similar with RNx Total RNA isolation kit (CinnaGen).

Authors and Affiliations

Javad Janpoor, Mohammad Farsi

Keywords

Related Articles

The effects of extrusion cooking on antinutritional factors, chemical propertiesand contaminating microorganisms of food

Extrusion cooking technology is a rapid processing method involving high temperature and pressure that is used to prepare a variety of processed foods. HTST processes are preferred because of the retention of nutrients...

Software Programs for analyzing genetic diversity

Initial selection and individual selection is basic in genetic diversity studies of vast populations. The selection can be done by some molecular techniques like molecular marker .This will be resulted in saving time a...

Effect of Salt and Drought Stresses on Seed Germination and Early Seedling Growth of Nepeta persica

High salinity in soil or irrigation water and drought are common environmental problems affecting seed germination and plant growth especially in arid and semi-arid regions. Seed germination is the most sensitive stage...

Performance evaluation of drip, surface and pitcher irrigation systems: A case study of prevalent urban landscape plant species

Urban landscape water use is undoubtedly one of the main water consumptions in arid and semi-arid regions. In this study, performance of surface, drip and pitcher irrigation systems on Ligustrum vulgare, Berberis thunb...

Effective DNA Extraction Protocol for Kenaf

Polymerase chain reaction (PCR) has a wide range of applications in molecular biological research. However, the quantity and quality of template DNA have a significant consequence on the reproducibility of PCR results....

Download PDF file
  • EP ID EP32372
  • DOI -
  • Views 312
  • Downloads 0

How To Cite

Javad Janpoor, Mohammad Farsi (2013). Assessment Of Total RNA Extraction From Edible Mushroom (Agaricus bisporus) With Three Current Methods. International Journal of Farming and Allied Sciences, 2(18), -. https://europub.co.uk/articles/-A-32372