Identification of Gaeumannomyces Graminis Varities by Specific Primers and Morphological Characteristics
Journal Title: International Journal of Farming and Allied Sciences - Year 2016, Vol 5, Issue 4
Abstract
Take-all disease caused by the fungus Gaeumannomyces graminis (Sacc.) Arx & Oliver var. tritici Walker is the most devastating root disease of cereals throughout the world. it has been also reported from different areas across Iran such as Kermanshah Province. During 2010-2011, three thousand four hudred twenty six diseased samples showing white head were collected from more than 300 wheat and barley fields visited in various parts of Kermanshah province. Pathogencity test of isoliated on wheat and oat was evaluated which all isolates causes disease on wheat. Based on morphological methods, all of isolates identified as G. graminis.var. tritici. Specific primers was used for identification of G. graminis . Two sets of primer pairs (NS5: GGT-RP and NS5: GGA-RP) were used to confirm identification and differentiation of the varities. This stage of testing showed that all isolates are G. g. var. tritici. The result also showed that G. g. var. avena wasn’t in collected isolates.
Authors and Affiliations
Mariam Yosefvand, Saeid Abbasi, Kianoosh Chagha-Mirza, Sohbat Bahram-Nezhad
Keywords
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