Lipopolysaccharide tolerance attenuates inflammatory responses by increasing hemeoxygenase 1 and tristetraprolin expression in Raw264.7 macrophages
Journal Title: Journal of Advanced Biotechnology and Experimental Therapeutics - Year 2018, Vol 1, Issue 1
Abstract
Inflammation is associated with the progression of a variety of diseases. Lipopolysaccharides (LPS) tolerance is recognized to reduce proinflammatory responses. Heme oxygenase1 (HO1) and tristetraprolin (TTP) are induced by LPS tolerance and mediate the antiinflammatory effects. However, it was not clear whether two molecules are linked in LPS tolerance. In this study, we sought to determine whether HO1 associates with TTP to mediate the anti-inflammatory effects of LPS tolerance. LPS treatment significantly increased mRNA and protein level of HO1 and TTP in a time dependant fashion while LPS significantly decreased mRNA and protein level of TNFα in Raw264.7 macrophages. LPS tolerance inhibited TNFα mRNA and protein while HO1 and TTP level was still increased. In HO1 deficient macrophages, LPS tolerance failed to attenuate TNFα mRNA expression but TTP level was still decreased. Our results suggest that HO1 and TTP are functionally linked in mediating anti-inflammatory effects of LPS tolerance. This novel LPS tolerance-HO1-TTP signaling pathway provides new possibilities for the treatment of inflammatory diseases.
Authors and Affiliations
Akhi Moni
Keywords
Related Articles
- EP ID EP36335
- DOI -
- Views 418
- Downloads 0
Analysis of telomere length in the coronary artery to determine the pathogenesis of coronary artery disease
Endothelial dysfunction is one of the earliest pathological features in atherosclerosis. The current study designed to analyse the telomere length of human coronary artery derived endothelial cells to identify the rela...
A reliable and easy method to isolate a pure population of bovine granulosa cells from slaughterhouse ovaries for in vitro studies
In a growing follicle, granulosa cells (GCs) are the major cellular component and perform several important functions. GCs are an excellent choice for in vitro experimentation to understand their functions in vivo. How...
Prophylactic effects of vitamin E and coriander (Coriandrum sativum) extract on lead-induced testicular damage in Swiss albino mice.
Lead toxicity is the vital issue in the developing countries that causes serious health hazards in animals and humans. Prophylactic effects of vitamin E and coriander (Coriandrum sativum) extract on lead-induced testic...
Expression patterns of the phosphoproteins and total proteins in TLQP-21 (a VGF derived peptide) treated SH-SY5Y cells
VGF (non-acronymic), belonging to a large granin family, gives rise to a number of bioactive peptides by proteolysis and exert an extensive array of biological effects on energy metabolism, pain modulation, gastric sec...
Prophylactic effects of vitamin E and coriander (Coriandrum sativum) extract on lead-induced testicular damage in Swiss albino mice
Lead toxicity is the vital issue in the developing countries that causes serious health hazards in animals and humans. Prophylactic effects of vitamin E and coriander (Coriandrum sativum) extract on lead-induced testic...