PENETAPAN KADAR PIPERIN DALAM EKSTRAK BUAH LADA HITAM (Piper nigrum Linn.) MENGGUNAKAN LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY (LC–MS/MS)
Journal Title: Jurnal Ilmiah Farmasi Farmasyifa (JIFF) - Year 2018, Vol 1, Issue 1
Abstract
The utilization of medicinal plants have started growing rapidly in the world, including in Indonesia, along with the thinking back to nature, one of the plants that are often used as a medicine is black pepper fruit (Piper nigrum Linn.). Piperine is a major compound and potent substances contained in black pepper fruit as antidiarrheal activity. The aim of this study was to determine levels of piperine in 96% ethanol extract of black pepper fruit. The extraction method used Soxhlet in 96% ethanol as solvent. Analysis of piperine using qualitative analysis by thin layer chromatography (TLC) produces yellow stain after being sprayed by dragendorf and dark blue on observation Uv-Vis 365 nm with Rf extract value 0.49 and Rf piperine value 0.5. Quantitative analysis using LC – MS produces piperine levels at 26%. Before quantitative analysis, validation method needs to be done with the parameters, among others: linearity have regression equation y = has 1,981,691.1333x + 561,445.0000 and correlation coefficient (R2) of 0.9973, LOD and LOQ at 0.14 and 0.44 ppm, accuracy value (% recovery) between 95.90 – 100.77%, precision value (% KV) between 0.02 – 1.84%, and selectivity (RT) between 2.47 – 2.49. The results of the validation parameters are eligible so that piperine levels were obtained by LC – MS declared accurate, specific, and precise.
Authors and Affiliations
Alifia Putri Febriyanti, Siti Jazimah Iswarin, Susanti Susanti
Keywords
Related Articles
- EP ID EP44735
- DOI -
- Views 388
- Downloads 0
POTENSI AKTIVITAS ANTIBAKTERI DARI FRAKSI ETIL ASETAT DAUN JAMBU AIR [Eugenia aqueum (Burn F.) Alston] TERHADAP STHAPHYLOCOCCUS AUREUS DAN ESCHERICHIA COLI
Staphylococcus aureus (S. Aureus) is a constituent of the Gram positive type of normal flora. But if the amount is excessive it will become a pathogen in humans. Likewise with Escherichia coli (E. coli) which is a gram n...
PENETAPAN KADAR PIPERIN DALAM EKSTRAK BUAH LADA HITAM (Piper nigrum Linn.) MENGGUNAKAN LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY (LC–MS/MS)
The utilization of medicinal plants have started growing rapidly in the world, including in Indonesia, along with the thinking back to nature, one of the plants that are often used as a medicine is black pepper fruit (Pi...
SUNSCREEN ACTIVITY OF SUGAR PALM (ARENGA PINNATA (WURMB) MERR.) LEAF STALK ASHES EXTRACT
Sugar palm leaf stalk (petioles) ashes traditionally used as a daily cosmetic by Sundanese women to keep their skin smooth. Sugar palm leaf stalk ashes also used to treat acne, smallpox, and burns. The study was aimed to...
UJI AKTIVITAS ANALGETIKA EKSTRAK n-HEKSANA DAUN AFRIKA (Vernonia amygdalina Delile) TERHADAP MENCIT SWISS WEBSTER JANTAN
Nyeri merupakan persepsi sensorik mengganggu yang dapat ditangani dengan analgetika. Daun afrika (Vernonia amygdalina Delile) secara tradisional digunakan untuk mengobati nyeri seperti sakit gigi. Tujuan penelitian ini u...
FORMULASI MINUMAN TEPUNG CACING TANAH (Lumbricus rubellus Hoffmeister) TERGANULASI DISERTAI UJI DAYA INGATNYA PADA MENCIT JANTAN
Protein merupakan suatu senyawa kimia yang penting bagi tubuh. Cacing tanah (Lumbricus rubellusHoffmeister) diketahui memiliki kandungan protein yang tinggi yang dapat dimanfaatkan sebagai protein alternatif. Penelitia...