Molecular and cytological evaluation of male sterile and restorer lines in hybrids rice
Journal Title: International Research Journal of Applied and Basic Sciences - Year 2012, Vol 3, Issue 1
Abstract
Information on the genetics of fertility restoration facilitates breeding and/or selection of restorer lines used in hybrid breeding program in a cytoplasmic male sterility (CMS) system. Cytoplasmic male sterility (CMS) is a common phenomenon that has been extensively used for production of hybrid seeds in various crops. Rf genes are needed for restoring fertility to CMS lines. Searching for molecular tagging of restorer genes is of high importance where phenotyping is very time consuming and requires the determination of spikelet sterility in testcross progeny. In order to determine maintainers and fertility restorers lines in hybrids rice, 5 cytoplasmic male sterile was crossed whit 10 pure line as tester. In other year, F1 progenies arranged in RCBD design whit three Replications. Evaluation of fertility in F1 populations showed IR-9 parent for all the crosses was identified as restorer. The cross Rome parent with CMS lines observed lowest amount fertility, also molecular analysis confirmed exist gene RF only in IR-9 parent.
Authors and Affiliations
Ammar Gholizadeh ghara*| M. Sc student, Science and Research branch, Islamic Azad University, Tehran, Iran.Ammar Gholizadeh Ghara, Email: ammar_gholizadeh@yahoo.com, Ghorbanali Nematzadeh| Department of Agronomy and Plant Breeding, Genetics and Agricultural Biotechnology Institute of Tabarestan, Sari Agricultural Science and Natural Resources University, Sari, Iran, Nadali Bagheri| Department of Agronomy and Plant Breeding, Genetics and Agricultural Biotechnology Institute of Tabarestan, Sari Agricultural Science and Natural Resources University, Sari, Iran, Asa Ebrahimi| Assistant Professor of Biotechnology, Science and Research branch, Islamic Azad University, Tehran, Iran., Morteza Oladi| Department of Agronomy and Plant Breeding, Genetics and Agricultural Biotechnology Institute of Tabarestan, Sari Agricultural Science and Natural Resources University, Sari, Iran
Civil liability in law of Iran and UK
Civil liability is the obligation of a person to compensation which the civil liability of another person without authorization from the legal right to occur when one of the loss to bring hurt, damage that may does not c...
Leisure time physical activity, playing digital games and health problems in youngsters
Background: Youngsters have great accessibility to computers. This affects the preferences of youngsters in terms of spending their free times. Playing digital games is one of the most popular activities for them, and th...
The Relationship between Conflict Management Styles and Effectiveness in Physical Educational Offices of Mazandaran Province
Conflict occurs when needs and desires of two individuals or parties are contradicting, consciously or unconsciously. In other words, conflict happens when parties are not getting what they want. Consequently, proper res...
Studying Affecting Factors on Customers’ Attitude toward Products with Halal Brand (Case study: Kuala lumpur, Malaysia)
Today, halal is not simply a religious issue rather it is an opportunity to increase sale and acquire competitive advantage. Concerning its importance, the aim of present research is to identify the beliefs that shape cu...
The Analysis of Reading Strategies used by Extrovert and Introvert Intermediate Students in ilam Province, Iran
This study investigates the relationship between extraversion/introversion personality dimension and the use of strategic competence (SC) in oral referential communication by Iranian FL learners. The ability to comprehen...