Protective effects of cordycepin on the histopathological changes and oxidative stress induced by hepatic ischemia/reperfusion in rats
Journal Title: Experimental Biomedical Research - Year 2018, Vol 1, Issue 1
Abstract
Aim: To investigate the effects of cordycepin on the histopathological changes and oxidative stress induced by hepatic ischemia/reperfusion (I/R) in rats. Method: Forty male Wistar rats were randomly divided into 4 groups as group I (sham, n=10), group II (control, n=10), group III (I/R-untreated, n=10) and group IV (I/R-cordycepin, n=10). Liver ischemia was induced for 30 min then reperfusion was allowed for 1 h. At the end of the experiment, liver specimens and blood samples were taken for histopathological and antioxidant evaluations, and biochemical analysis. Results: The levels of IL6, IL-1β, and TNFα in the serum and liver tissues were higher in the I/R-untreated group compared to the I/R-cordycepin treated group. In the I/R-cordycepin group, serum MDA levels were decreased compared with the I/R-untreated group. The I/R-cordycepin treated group showed an increase in TAS levels, and a decrease in TOS levels compared with I/R-untreated group. The histopathological injury score were significantly lower in the I/R-cordycepin treated group compared to the I/R-untreated group. In the I/R-untreated group, the integrity of the hepatocyte cell lines deteriorated. Mononuclear inflammatory cells infiltrated the parenchyma regions, the sinuses dilated and there was diffuse congestion Preoperative treatment with cordycepine reduced histopathological abnormalities.
Authors and Affiliations
Mehmet Hanifi Okur, Hikmet Zeytun, Erol Basuguy, Gulay Aydogdu, Ulas Alabalık, Ibrahim Kaplan, Sevinc Akdeniz, Serkan Arslan, Bahattin Aydoğdu
Keywords
Related Articles
- EP ID EP50798
- DOI https://doi.org/10.30714/j-ebr.2018136920
- Views 264
- Downloads 0
Investigation of variants of critically important antioxidant enzyme genes in patients with polycystic ovary syndrome
Aim: To investigate the possible effects of polymorphisms in genes encoding some important antioxidant enzymes such as super oxide dismutase 2 (SOD2), glutathione peroxidase 1 (GPX1), endothelial NOS (eNOS) and catalase...
The relation between antiepileptic drug type and cognitive functions in childhood epilepsy: A prospective observational study
Aim: This study investigated the effect of antiepileptic drug monotherapy on cognitive functions in pediatric epilepsy patients. Methods: 98 recently diagnosed epilepsy patients aged 6-16 years were included. All pati...
Patients’ misperception regarding the difficulty of lumbar puncture
Aim: Lumbar puncture (LP) is a crucial method of diagnosis and treatment of neurological diseases. Despite its importance, the patients' refusal of the procedure leads to difficulties in diagnosis and treatment. One of t...
Our experience regarding withdraw blood from rats and preparation of platelet-rich plasma
Aim: Withdraw blood from rats is an important, but not easy, invasive procedure in experimental research on these animals. In addition, the preparation and standardization of platelet-rich plasma (PRP) is a more difficul...
Thyroid fine needle aspiration reporting rates and outcomes before and after Bethesda implementation: A single-center experience over 8 years
Aimː To evaluate data from our hospital system before and after the implementation of the Bethesda System for Reporting Thyroid Cytology (TBSRTC) and comparison of our data with the previously published studies. Metho...