Effects of Low-Energy Laser Irradiation on Sperm Cells Dynamics of Rabbit (Oryctolagus Cuniculus)
Journal Title: Journal of Nuclear Physics, Material Sciences, Radiation and Applications - Year 2017, Vol 5, Issue 1
Abstract
Infertility is a world disease in which a couple is unable to achieve pregnancy. There are numerous parameters to determinate fertility; nevertheless, sperm motility is by consensus one of the most important attributes to evaluate male fertility. Contributions to a better understanding of this crucial parameter are imperative; hence, the aim of this investigation was to assess the effect of low-energy laser irradiation on sperm cell dynamics in thawed samples that were cryopreserved. We used a 405 nm blue laser beam to irradiate spermatic cells from rabbit inside a temperature-controlled dispersion chamber at 37 °C; then, we applied an image recognizing system to calculate individual sperm trajectories and velocities. We found that sperms raise its motility after irradiation suggesting that λ=405nm is an optimal wavelength for spermatic photo-stimulation.
Authors and Affiliations
J. M. De Jesus-Miranda, L. A. Mandujano, F. Mendez, Y. J. Castillo, J. Mulia, C. Garcia, Y. E. Felipe, D. Osorio-Gonzalez
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