Lactic Acid Synthesis by Fungus Rhizopus oryzae F-1030 on Growth Media Based on Sulphite Liquors
Journal Title: Lesnoy Zhurnal (Russian Forestry Journal) - Year 2020, Vol 13, Issue 2
Abstract
Lactic acid is an industrially important product with an expanding consumer market. However, lactic acid production and isolation methods used at the present time are not effective enough, lead to the formation of large amounts of polluting waste and their recycling is economically unprofitable. At the same time, a half of the worldʼs lactic acid production is carried out by the microbiological method based on the fermentation of such costly sugarcontaining substrates as sucrose, molasses, treacle, sugar syrup, etc. These sugar-containing substrates usage significantly increases the final product cost. In order to solve the conomic and environmental problems of lactic acid production it is necessary to revise the current raw material source and put cheaper and readily available sources of carbohydrates, such as sulphite liquor formed during sulphite pulping, into the lactic acid production. In turn to enhance the economic efficiency of the Russian pulp and paper production it is necessary to use such paper production by-products as sulphite liquor to the fullest extent possible. Sulphite liquor is a chemical complex of inorganic and organic compounds such as monoand oligosaccharides. The article considers the dependence of the output of lactic acid synthesized on the sulphite liquor medium by the fingus R. oryzae F-1030 on the used method of cultivation. In case of the semicontinuous culture method, the culture liquid was replaced with the similar volume of the sterile growth medium with the fungus biomass saving when the sugars in the medium were depleted. In case of the batch culture method, the synthesized lactic acid was precipitated with calcium hydroxide and the reducing substances recovery in the culture liquid was achieved by adding concentrated sulphite liquor when the sugars in the medium were depleted. The study demonstrates that the largest amount of synthesized lactic acid is obtained when using the semicontinuous method for cultivation of the fingus on the sulphite liquor medium prepared according to the technology recommended in the industry during preparation growth media for yeast cultivation. If it is impossible to carry out a full industrial pre-treatment of sulphite liquor, it is recommended to use the batch culture method for the fungus in order to obtain more synthesized lactic acid.
Authors and Affiliations
L. A. Mingazova, A. V. Kanarsky, E. V. Kryakunova, Z. A. Kanarskaya
Keywords
Related Articles
- EP ID EP683266
- DOI 10.37482/0536-1036-2020-2-146-158
- Views 260
- Downloads 0
Breeding Tests of Juvenile Factorial Hybrids of Populus tremula L.
The aspen is known for its rapid growth, unpretentiousness to growing conditions, valuable timber and some other useful properties. The largest areas on the Eurasian continent are occupied by Populus tremula L. Unfortuna...
The Use of Nanosized ZnO in Compositions for Wood Protective Treatment
Wood natural structure can be considered as a suitable matrix for modifying with nanoparticles of various chemical nature. The research aims at obtaining a woodbased nanocomposite by modifying wood with compositions of w...
Study of Kraft Lignin Nitration with Acetyl Nitrate in the Presence of Aprotic Solvents
A method for homogeneous nitration of kraft lignin (KL), which consists in its treatment with acetyl nitrate in 1,4-dioxane, tetrahydrofuran, or dimethyl sulfoxide, has been proposed. The effects of reaction time and con...
Evaluation of the Accuracy of Wood Drilling Resistance Measurement Method in Dendrochronological Studies
One of the promising methods for determining physical and mechanical properties and structure of wood is the method based on the measurement of drilling resistance. Limitations of application of the method of measuring d...
Climate Change and Its Impact on Adaptation and Intraspecific Variability of Conifer Species of the European North of Russia
Current climate change is affecting forests and requires a specific forest management strategy. The review aims to analyze the impact of observed and projected climate change on the tree species adaptation response with...