Detection and quantification of quitinasa in banana (Musa spp.) leaves inoculated with Mycosphaerella fijiensis
Journal Title: Biotecnología Vegetal - Year 2012, Vol 12, Issue 2
Abstract
The knowledge of the role of chitinase enzyme in Musa spp- Mycosphaerella fijiensis interaction is still very limited. Therefore, their study is of great interest, mainly in breeding programs. The aim of this study was to detect and quantify chitinase enzyme in leaves of ‘Grande naine’ and ‘Calcutta 4’ inoculated with M. fijiensis in greenhouse conditions. This enzyme accumulation was detected in leaves of both genotypes and at different times after inoculation using agarose gel with glycol chitin as a substrate and it was revealed by using Calcoflour brightener. It was noted that in ‘Calcutta 4’ plants there was an increase in chitinase activity in inoculated compared to non-inoculated plants at all times studied. In ‘Grande naine’ inoculated plants this difference was only observed at 2 h and at 3 days after inoculation respect to non-inoculated plants. At each time, the highest values of enzyme concentration corresponded to samples of the resistant genotype. It was shown that the accumulation of this enzyme was related to plant response to pathogen inoculation and was higher in inoculated plants of resistant genotype.
Authors and Affiliations
Cynthia Sánchez-García,* Mileidy Cruz-Martín, Yelenys Alvarado-Capó, Luis Rojas, Michel Leiva- Mora, Mayra Acosta-Suárez, Berkis Roque
Keywords
Related Articles
- EP ID EP683716
- DOI -
- Views 203
- Downloads 0
Obtaining of microtubers of potato cv. ‘Andinita’ in Temporary Immersion Systems
The Instituto Nacional de Investigaciones Agrícolas (INIA), Venezuela, is producing potato seed by biotechnology in its National Seed Plan. The seeds needed are greater than the installed capacity. The acquisition of t...
Effect of different disinfection methods on the in vitro establishment of Guadua angustifolia Kunth
The bamboo is a plant that possesses multiple uses with an enormous economic, social, cultural and environmental impact. The present research work was developed with the objective of determining the effect of different d...
Ef fect of the inoculation of phospha te solubilizing microorganisms and arbuscular mycorrhizal fungi in Eugenia dysenterica plants grown on different substrates
Phosphate solubilizing microorganisms (PSM) and arbuscular mycorrhizal fungi (AMF) represent alternative strategies to reduce the cost and increase the production of cagaita (Eugenia dysenterica) seedlings associated wit...
Effect of Sodium Chloride on the in vitro development of cultivars 'Gran enano' and 'FHIA 03'
The effect of Sodium Chloride levels on in vitro development of Gran Enano (AAA) and `FHIA 03`(AABB) cultivars was determined. Researches were carried out at Instituto de Investigaciones en Viandas Tropicales (INIVIT)...
In vitro propagation of Vriesea in immersion systems temporary
Bromeliads are ornamentals plants very attractive due to leaves coloration and the beautiful inflorescences. Vrieseas is a genus of a great commercial interest among them. Traditional propagation techniques are limited...