Field evaluation of inactivated Corynebacterium ovis vaccine in sheep prepared from a local isolate
Journal Title: UNKNOWN - Year 2016, Vol 23, Issue 1
Abstract
Corynebacterium pseudotuberculosis vaccine was prepared from a local field isolate. Vaccination of sheep with 50g PLD toxoid and 10 mg bacterin adjuvanted with Montanide oil improved the levels of immune responses of sheep. In many countries, inactivated C. pseudotuberculosis adjuvant vaccines have been used for prevention and control of caseous lymphadenitis in sheep. However, the efficacy was variable. The aim of the present study was directed to prepare and evaluate the potency of an inactivated C. pseudotuberculosis vaccine using Montanide ISA206. Sheep were vaccinated with 1st dose of 2ml containing 10 mg bacterin and 50g toxoid and Montanide ISA 206 oil adjuvant and boostered with the same dose 15 days Apart. Evaluation of post vaccinal cellular immune response with lymphocyte proliferation assay and humoral immune response using ELISA was carried out. Cell mediated immune response of vaccinated sheep reached its peak 0.445 by 1st week post the second vaccination. The level of humoral immune response showed optical density of 1.005 by 1st week post the second vaccination. Challenge test was done in all sheep four weeks after the second dose of vaccination. Three sheep from vaccinated and three sheep from non-vaccinated groups were slaughtered and necropsied 150 days post challenge. The results revealed 75% protection percentage against challenge while unvaccinated challenged sheep showed 9% protection. Statistical analysis indicated that the vaccine assessed a significant level of cellular and humoral immunity.
Authors and Affiliations
Nabila A. Ghazya, Mohamed G. Abdelwahabb, Makhareta M. A. M. c, and Abdallah Y. A. d a Department of Serum and Antigens, Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt. b Department of Animal Medicine, Faculty of Veterinary Medicine, Benha University, Egypt. c Central Laboratory for Evaluation of Veterinary Biologics, Abbassia, Cairo , Egypt. d Department of Anaerobic Vaccines, Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt.
Keywords
Related Articles
- EP ID EP95166
- DOI 10.21608/jvmr.2016.43229
- Views 178
- Downloads 0
EFFECT OF VIRGIN OLIVE OIL SUPPLEMENTATION ON LIPID PROFILE AND OXIDATIVE STATUS IN RATS
The aim of the present study is to investigate the effect of virgin olive oil on some blood parameters in male Albino rats supplemented with normal diet. thirty male Sprague Dawley rats, (90-110 g), were used in the pr...
Physicochemical and bacteriological status of retail-marketed shrimps and crabs in Beni-Suef, Egypt
A total of a hundred and twenty samples of crustaceans, 60 samples of shrimp (30 peeled & 30 unpeeled shrimps) and 60 samples of local chilled crab were collected from Beni-Suef markets. Collected samples were evaluate...
Morphological pathology of bovine ovarian abnormalities in correlation to uterine changes
Female genital tracts of six to eight years Baladi cows (n=30) were collected from Belevia abattoir of Beni-Suef province during the period from March 2016 to May 2016 to investigate ovarian abnormalities and uterine c...
Risk assessment of some residues in milk and some dairy products
Milk and dairy products are an important exposure route for organochlorine pesticides (OCPs) to humans. For this reason milk, kareish cheese and yoghurt analyzed for the presence of fourteen organochlorine pesticides...
Microbiological risk assessment in ready to eat processed meat
A total of (120) sample of ready to eat (RTE) meat;(20) each of cooked luncheon, frankfurter, hot dog, pasterma, shawrma and smoked luncheon were collected from different shops and grocery stores in Beni-Suef City. To...