Adventitious Bud Formation and Plantlet Regeneration of Achyrocline satureoides-A Multipurpose Medicinal Plant
Journal Title: European Journal of Medicinal Plants - Year 2014, Vol 4, Issue 10
Abstract
Adventitious bud regeneration was achieved from hypocotyls, cotyledons and leaf explants of Achyrocline satureioides. Organogenesis was induced from every explant cultured on Murashige and Skoog semisolid medium (plus sucrose 30g•L-1) containing different combinations of 6-benzyladenine (BA) and -naphtalenacetic acid (NAA) under 116mol•m2•s-1 photosynthetic photon flux density (PPFD), photoperiod 14 h and at 272ºC. The regeneration was similar for every tested explant and varied between 64 and 83%. The number of buds formed per regenerative explants was similar in every treatment (5-8 shoots/explant). In order to stimulate In vitro rooting, regenerative leaves were sub cultured from the best induction medium in MS lacking plant growth regulators for the same periods. Every plantlet raised In vitro was phenotypically normal and successfully hardened to ex vitro conditions. An experimental field plot with 60-day-old in vitro regenerated plants was established.
Authors and Affiliations
D. Kotik, P. Sansberro, C. Luna
Keywords
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- EP ID EP350258
- DOI 10.9734/EJMP/2014/8800
- Views 115
- Downloads 0
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