Antimalarial Activity of Crude Extract of Buddleja Polystachya Fresen (Buddlejacea) Against Plasmodium Berghei in Mice
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2016, Vol 11, Issue 5
Abstract
Malaria is one of the most serious widespread diseases encountered by human and it is the second leading cause of death from infectious diseases next to HIV/AIDS in Africa. People, in several parts of the world, use different traditional medicines for treating malaria and it has been reported that the roots, leaves, and flowers of various species of Buddleja are used for the same purpose. The aim of the present study was, therefore, to evaluate in vivo and phytochemical screening of secondary metabolites, safety and antimalarial activity of the crude extractof Buddleja polystachy in mice. The animals were divided into 5 groups. The first group served as negative control and was administered with vehicle (0.2ml of distilled water). Group two (B100), three (B200) and four (B300) were administered 100, 200 and 300 mg/kg Buddleja polystachy extract, respectively. Group five served as positive control group and was administered with chloroquine (10mg/kg/day). Antimalarial activity was evaluated using 4-day suppressive assays against P. Berghei by orally administering the mice with either vehicle, various doses of the Buddleja polystachya extract or chloroquine, while safety study was performed using acute toxicity studies by administering 2000mg/kg of the Buddleja polystachya extract to non-infected mice.The effect of crude extract was was measured and analyzed using One-way ANOVA and paired t-test. The result showed that the extracts did not caused symptoms of toxicity at the given doses. Preliminary phytochemical screening of the leaves powder of the plant indicated the presence of major antimalarial secondary metabolites. The extract of Buddleja polystachya leaves exerted dose dependent parasitemia suppression, although the active responsible principles are yet to be identified, which need further studies to elucidate the antimalarial mechanism of their action. It can be concluded that crude extracts of Buddleja polystachya showed strong activities against P. berghei indicating that it contains some chemical constituents that possibly lead to antimalarial drug development
Authors and Affiliations
Nurahmed Mohammed, , Mohammedberhan Abdulwuhab, Faiz Mohammed
Keywords
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- EP ID EP385917
- DOI 10.9790/3008-1105012735
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