Antioxidant and Cytotoxic Activity of Water Ethanol Extracts from the Mycelium of Inonotus rheades (Pers.) Bondartsev & Singer
Journal Title: Журнал ÑтреÑÑ-физиологии и биохимии - Year 2016, Vol 12, Issue 4
Abstract
The important problem of current research is the search for bioactive natural compounds. We have previously demonstrated high [i]in vitro[/i] antiradical activity of water ethanol extracts (WEE) from the mycelium species of the genus Inonotus rheades. In the present study we evaluated the effect of 30% and 70% of WEE from mycelium [i]I. rheades[/i] on the cell culture of human tumor cells HEp-2. 50% cell death is achieved after incubation in 53 mkg/ml 70% WEE (dry weight), whereas 30% WEE at 550 mkg/ml only, i.e. an order of magnitude difference between the concentrations, which indicates that high cytotoxic activity was shown WEE a radical change in the qualitative composition of extractives with increasing ethanol concentration. Incubation of cells with 250 mM H[sub]2[/sub]O[sub]2[/sub] resulted in approximately 40% cell death, whereas pre-incubation with both 30% and 70% of WEE resulted in 100% survival of cells in a large range of concentrations. This is indicate a WEE protective effect under oxidative stress. In the case of simultaneous incubation of the cells with 30% and 70% WEE and H[sub]2[/sub]O[sub]2[/sub] the effect was different with described earlier. Complete cell survival was not achieved at all concentrations of the extract of mushroom. However, significant dilution of WEE leads to significantly protective antioxidant effect on HEp-2. Thus, water ethanol extracts of the mycelium [i]I. rheades[/i] in high concentrations exhibit cytotoxic activity. At the same time, a wide range of concentrations extracts can neutralize the oxidative stress and cell death caused by the addition of hydrogen peroxide when making extracts to oxidative influences.
Authors and Affiliations
M. Borovskaya, T. Gornostay, G. Borovskii
Keywords
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