ANTIOXIDANT AND HEPATOPROTECTIVE ACTIVITIES OF LIBIDIBIA FERREA BARK AND FRUIT EXTRACTS
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2014, Vol 6, Issue 11
Abstract
Objective: The aim of this study was to investigate the antioxidant and hepatoprotective activities of Libidibia ferrea bark and fruit extracts.Methods: The extracts were analyzed by high performance liquid chromatography. Antioxidant potential was studied using DPPH, ABTS, superoxide anion radical, β-carotene and cellular assay. The extracts were also evaluated for hepatoprotective activity against carbon tetrachloride (CCl4) induced acute liver injury in mice.Results: Both fruit and bark extracts demonstrated high free radical scavenger activities using DPPH or ABTS assays and superoxide anion radical tests when compared with standards. Extracts of L. ferrea also demonstrated antioxidant activity in a cell-based assay. In this assay, bark extract showed better activity than fruit extract (more than 60% inhibitions at extract concentration 50 µg/mL). Extracts significantly prevented the CCl4 induced hepatic damage as indicated by serum marker enzymes (GOT, GPT and ALP). L. ferrea extracts also prevented CCl4 induced oxidative stress by inhibiting lipid peroxidation. Both bark and fruit extracts showed substantial antioxidant activity explained by the presence of polyphenol compounds like gallic and epicatechin. The administration of bark and fruit extracts from L. ferrea prevented liver biochemical alteration induced by CCl4.Conclusion: The extracts of L. ferrea possess free radical scavenging activity under in vitro conditions and cell-based assay, and could protect the liver against CCl4 induced oxidative stress by increasing antioxidant defense activities.Â
Authors and Affiliations
Aline Oliveira Barros, Rodrigo Silva De Souza, Elenn Suzany Pereira Aranha, Leydiana Moraes Da Costa, Tatiane Pereira De Souza, Marne Carvalho De Vasconcellos, Emerson Silva Lima
Keywords
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