Characterization of Methicillin Resistant Staphylococcus aureus based on its virulence factors and antimicrobial susceptibility profile
Journal Title: Indian Journal of Microbiology Research - Year 2017, Vol 4, Issue 1
Abstract
Staphylococcus aureus remains as one of the most potent bacterial human pathogen because of its expression of various virulence factors and also due to its property of multidrug resistance. A total of 468 non –duplicate S.aureus strains obtained from various clinical specimens were included in the study. Methicillin Resistant S.aureus (MRSA) strains were screened for DNase production using DNase agar, hemolytic property of the isolates were detected in 5% sheep blood agar plates, hemagglutination property of the isolates were demonstrated using 1% O group RBC’s,slime production was detected using congo red agar medium and biofilm production was quantitatively assayed by microtitre plate method. Antimicrobial susceptibility profile was studied for the MRSA isolates. Out of 468 S.aureus strains, 114(24%) strains were detected as MRSA. Among the MRSA strains 99(86%) were positive for DNase, 77(67%) were showed beta hemolysis, 49 (42%) strains were positive for hemagglutination, 70 (61%) were slime producers, most of the strains were biofilm producers, 4(3.5%) were non-adherent, 85(74%) were weakly adherent, 23(20%) were moderately adherent and 2(1.7%) were strongly adherent. About 56%, 60% and 63% of the isolates were resistant to cotrimoxazole, erythromycin and ciprofloxacin respectively and about 22% and 33% of the isolates showed resistance towards clindamycin and gentamicin respectively. All the isolates were sensitive to vancomycin, teicoplanin and linezolid. About 10(8.7%) strains showed high level mupirocin resistance (HLMR) and 1(0.8%) strain showed low level mupirocin resistance (LLMR). Both HLMR and LLMR (100%) strains showed susceptibility to fusidic acid. MRSA infections remain a major threat in both community and nosocomial settings. Therefore a thorough understanding of its virulence mechanisms and regular surveillance of antimicrobial susceptibility pattern will help the clinician to choose appropriate treatment options and to control the emergence of multidrug resistant strains.
Authors and Affiliations
Alice Peace Selvabai, A. S. Shameem Banu, M. Jeya, Priyadarshini Shanmugam
Keywords
Related Articles
- EP ID EP293920
- DOI 10.18231/2394-5478 .2017.0015
- Views 76
- Downloads 0
Prevalence and antibiotic susceptibility of neisseria gonorrhoeae in HIV positive and hiv negative cases at a tertiary care hospital
Introduction: Gonorrhea is one of the most common sexually transmitted infections (STIs) in developing countries, and the emergence of resistance to antimicrobial agents in Neisseria gonorrhoeae is a major hindrance in t...
Bacterial profile and antibiotic susceptibility in chronic obstructive pulmonary disease patients with acute exacerbation: A cross sectional study in a tertiary care hospital
Background: The course of Chronic Obstructive Pulmonary Disease (COPD) is characterized by intermittent exacerbations of the disease. The bacterial pathogens contribute a lot in mucus secretions and the acute exacerbatio...
Comparative evaluation of a rapid test with ELISA for the detection of Dengue Infection
Background: Dengue is a global health issue. The clinical illness ranges from an asymptomatic febrile illness to dengue haemorrhagic fever (DHF) and dengue shock syndrome (DSS). Objectives: The present study was undertak...
Study of antimicrobial resistance pattern in blood isolates from critical care unit at a Tertiary Care hospital, Udaipur, Rajasthan
Introduction Microorganisms present in circulating blood are a threat to the every organ of the body Blood stream infections can have serious consequences and timely detection and identification of blood stream pathogen...
Current concepts of diagnosis for mycobacterial infections in female genital tract
Female genital tuberculosis is a very common cause of infertility not only in India but in other developing countries also. The organ which gets most affected is fallopian tubes (90-100%), followed by endometrium (50-60%...