Cytotoxic Effect of Ethanolic Extract of Sarang Semut (Myrmecodia pendens) on HeLa Cervix Cancer Cell Line In Vitro Experimental Study
Journal Title: SAINS MEDIKA (Journal of Medicine and Health) - Year 2011, Vol 3, Issue 2
Abstract
Background: Sarang semut (Myrmecodia pendens) is a traditional plant that has been known to contain anti cancer components. Flavonoids and tannins were contained in sarang semut plant which are believed has cytotoxic effect against cancer cell line. This study aims at cytotoxic effect ethanolic extract of sarang semut at various concentrations on HeLa cervical cancer cell line. Design and Method: The method was quasi experimental with post test only non equivalent control group design. HeLa cell was divided into two groups. The first group as positive control with doxorubicin, second group as treatment with ethanolic extract of sarang semut at various concentrations. Ethanolic extract of sarang semut concentrations used were 3,91 μg/ml; 7,81 μg/ml; 15,63 μg/ml; 31,25 μg/ml; 62,50 μg/ml; 125 μg/ml; 250 μg/ml; 500 μg/ml; 1000 μg/ml. Cytotoxic effect was evaluated by direct counting method with tryphan blue dye then using probit regression analysis to find IC50 value. Result: Inhibitory concentration 50 (IC50) value ethanol extract of sarang semut was 33,28 μg/ml. Ethanol extract of sarang semut had a cytotoxicity effect categorized as the moderately active (20 ìg/ml< IC50< 100ìg/ ml). Inhibitory concentration 50 (IC50) value doxorubicin was 5,56 μg/ml. Cytotoxicity effect of doxorubisin higher than cytotoxicity effect of ethanolic extract of sarang semut. Conclusion: Ethanolic extract of sarang semut (Myrmecodia pendens) had a cytotoxic effect categorized as the moderately active on HeLa cell (Sains Medika, 3(2):112-120).
Authors and Affiliations
Dina Fatmawati, Prista Karina Puspitasari, Iwang Yusuf
Keywords
Related Articles
- EP ID EP238169
- DOI -
- Views 95
- Downloads 0
The Length of Stay Difference between Under 5 Years Patient with Acute Diarrhoea Treated With and Without Probiotic An Analytical Study in RSUD Kota Semarang during the Period January to December 2007
Background: Diarrhoea is still public health problem and often cause outbreak with various morbidity and mortality. The long onset of acute diarrhoea causes the length of stay that affect the cost. So proper management i...
The Role of Vitamin in the Nutrition of Premature Infants
Vitamin is an organic compound required as a vital nutrient in tiny amounts. Premature infants are at risk of vitamin deficiency than mature infants, due to inadequate transplacental transport, inadequate storage, and in...
A Child with Tethered Cord Syndrome
Tethered cord syndrome mengacu pada sejumlah gangguan neurologis yang berkaitan dengan malformasi medulla spinalis, berupa tertariknya medulla spinalis ke basis canalis spinalis. Tethered cord syndrome dapat ditemukan pa...
Antioxidant Activity of The Crude Carotenoid Pigment Extract from Yellow Ambon Banana (M. parasidiaca sapientum) Peel: Its Potency as Vitamin A Supplement
Background: Carotenoid pigment is one of the promising sources of provitamin A because it has been shown to have capacity to be converted into vitamin A by the body. This present study aimed at evaluating the antioxidant...
Effect of Ginger Juice Water on Blood Glucose Levels and Histopathology of Pancreatic Beta Cells Experimental Studies on Alloxan Induced Wistar Male Rats
Background: Hyperglycemia is one of the signs of diabetes mellitus, the disease is still being suffered by the people of Indonesia. Oral antidiabetic drugs mostly give unwanted side effects, then the experts developed a...