Detection of Hepatitis E Virus RNA in Chicken Droppings and Pig Feces in Ogun and Lagos States, South Western, Nigeria
Journal Title: Journal of Advances in Medicine and Medical Research - Year 2016, Vol 12, Issue 4
Abstract
Background/Aim: Hepatitis E virus (HEV) is a major cause of sporadic and epidemic cases of enteric non-A non-B hepatitis in humans. It estimated that 14 million symptomatic cases of HEV infection, with 300,000 deaths and 5,200 stillbirths occur annually worldwide, with developing countries in the Indian subcontinent, Southeast and Central Asia, the Middle East, and northern and western parts of Africa being the most affected. This study was carried out to detect the presence of HEV RNA in commercial chicken and pigs in some parts of Ogun and Lagos states, South Western, Nigeria. Materials and Methods: A total of 550 fecal samples were collected from chicken and pigs in both states. HEV RNA was extracted from the fecal samples and amplified by nested-PCR. Gel electrophoresis was used to evaluate the nested-PCR products. Results: HEV RNA was detected in 10(1.8%) of the 550 samples. This comprised of 5(6.7%) positive from chicken droppings and 5(1.7%) from pigs feces. The result also showed that 3(4.3%) of chicken droppings collected from Ogun state were positive while there was no positive cases recorded in pig feces. Similarly 2(40%) of chicken droppings collected in Lagos state were positive while 5(1.7%) of pig feces were also positive. Conclusion: The detection of HEV among commercially available chicken and pigs poses a great economic danger to poultry farmers and a tremendous public health risk to consumers of pork meat in Nigeria.
Authors and Affiliations
E. E. Ogbolu, R. K. Obi
Keywords
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- EP ID EP339923
- DOI 10.9734/BJMMR/2016/15467
- Views 55
- Downloads 0
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