DEVELOPMENT AND VALIDATION OF FIRST ORDER DERIVATIVE UV SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS ESTIMATION OF PROPRANOLOL HYDROCHLORIDE AND FLUNARIZINE DIHYDROCHLORIDE IN BULK AND COMBINED DOSAGE FORM
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2013, Vol 4, Issue 6
Abstract
The first order derivative of UV spectrometry method for simultaneous determination of Propranolol hydrochloride (PRO) and Flunarizine dihydrochloride (FLU) in pure bulk drug and combined dosage form was found to be simple, accurate, fast, precise and reproducible. The first derivative values measured at 289nm for PRO and 253nm for FLU. The linearity for zero order derivative method was carried out by using the concentration range 4-28µg/ml for PRO and 3-7µg/ml for FLU. The coefficient correlation of PRO and FLU for zero order was found to be 0.9995 and 0.9991 respectively. At zero crossing point of PRO (289nm) FLU showed a measurable derivative absorbance where as at the zero crossing point of FLU (253nm), PRO showed appreciable derivative absorbance value. The coefficient correlation of PRO and FLU for first order derivative was found to be 0.9991 and 0.9995 respectively. Precision study showed that % RSD was within the range of acceptable limits (<2). The % recovery for PRO and FLU was found to be in the range of 98-102% and 100-101% respectively. The percentage assay was found to be as 99.5 and 100.12% for PRO and FLU. The results of analysis have been validated as per ICH Q2 (R1) guidelines. This method has applied successfully for the determination of PRO and FLU in its combination with a high percentage of recovery good accuracy and precision.
Authors and Affiliations
Dipmala Wagh , Vishakha Deshmukh , Swetal Vassa , Hemant Jain
Keywords
Related Articles
- EP ID EP135870
- DOI 10.7897/2230-8407.04639
- Views 94
- Downloads 0
A REVIEW ON SYNTHESIS OF SOME N-(p-METHOXY BENZENE SULPHONYL) GLUTAMAMIDE
Numerous studies on glutamine metabolism in cancer demonstrate that glutamic acid and glutamine analogues may be developed as possible antitumor agent. Efforts were made to synthesize glutamamide analogues, structure var...
TECHNOLOGY TRANSFER IN PHARMACEUTICALS
The Purpose of this review article is to discuss various critical paths for success of technology transfer. This article highlights the goal, methods, importance, facets and various models associated with technology tran...
COMPARATIVE STUDY OF IN-PROCESS AND FINISHED PRODUCTS QUALITY CONTROL TESTS OF INDIAN PHARMACOPOEIA, BRITISH PHARMACOPOEIA & UNITED STATES PHARMACOPOEIA FOR CAPSULES AND LIQUID ORALS
Present study deals with a brief overview of the comparative study of quality requirements for in-process and finished products quality control Tests of Indian Pharmacopeia (IP), British Pharmacopeia (BP) & United St...
OPTIMIZING ORAL DRUG DELIVERY USING LIPID BASED FORMULATIONS
A great challenge facing the pharmaceutical scientists is transforming the new pharmacologically active lipophilic compounds that are poorly water soluble into orally administered medications with sufficient bioavailabil...
EFFICACY OF KSHARSUTRA IN THE MANAGEMENT OF CHRONIC FISSURE IN ANO
The aim of the present study was to evaluate the role of ksharsutra ligation in the management of chronic fissure in ano. Ksharsutra has been in use in the management of fistula in ano successfully and the same has been...