Development of an Efficient Plant Regeneration System of Field Mustard (Brassica campestris)
Journal Title: Biotechnology Journal International - Year 2016, Vol 10, Issue 2
Abstract
Aims: The present study was conducted with a view to develop an efficient protocol for high frequency plant regeneration of Brassica campestris for further crop improvement program by biotechnological manipulation and to optimize this system for regeneration of a number of B. campestris genotypes. Study Design: Completely Randomized Design. Place and Duration of Study: This experiment was carried out in the Genetic Engineering Laboratory of the Department of Genetics and Plant Breeding, Sylhet Agricultural University, Bangladesh during the period of July 2013 to June 2014. Methodology: Cotyledon and hypocotyl explants of B. campestris cv. BARI sarisha-12 were cultured on MS medium supplemented with different concentrations of 6-Benzylaminopurine (BAP) and α-Naphthaleneacetic acid (NAA) for callus initiation and shoot regeneration. Later on subsequent subculturing is done for shoot elongation and multiplication. MS medium supplemented with various concentrations of NAA were used for root formation. Results: From a total of 15 different combinations of BAP and NAA tested, the combination of 1.0 mg L-1 BAP and 0.5 mg L-1 NAA gave the highest frequency of callus initiation (94.44%) as well as shoot regeneration (63.89%) in case of cotyledon explants where as hypocotyl explants showed 47.62% callus initiation and 19.04% shoot regeneration frequency. Four days old cotyledon explants showed the highest shoot regeneration frequency (72.22%) and higher number of shoots per explant (3.94) than those from older seedling. The shoot regeneration frequency markedly enhanced to 83.33% by the addition of 2.0 mg L-1 AgNO3 to the MS medium supplemented with 1.0 mg L-1 BAP, 0.5 mg L-1 NAA and this combination also showed the maximum number of shoots per explant (6.86). Shoot regeneration potentiality of five B. campestris genotypes were investigated and indicated that this system would be widely applicable to all the genotypes. The regenerated shoots were easily rooted on MS medium supplemented with 0.2 mg L-1 NAA and the whole plants were transferred to pot soils and grown to maturity. Conclusion: MS medium supplemented with 1.0 mg L-1 BAP, 0.5 mg L-1 NAA and 2.0 mg L-1 AgNO3 is more efficient for multiple shoot regeneration by using cotyledon explants and it may be utilized for In vitro improvement program of B. campestris.
Authors and Affiliations
Mohammed Shafi Ullah Bhuiyan, Kishore Kumar Sarker, Fakhrul Islam Monshi, Sayeda Sultana, Delara Akhter
Keywords
Related Articles
- EP ID EP237684
- DOI 10.9734/BBJ/2016/21529
- Views 80
- Downloads 0
Frequency of Procedural Errors during Root Canal Treatment Performed by Interns
Aims: Chemo-mechanical preparation continues to be one of the most challenging steps in root canal treatment procedures. The aim of this study was to examine the frequency of procedural errors during root canal treatment...
Kerosene: A Study of Serum Vitamin Levels of Female Wistar Rats Chronically Exposed
Background: Kerosene is a commonly available product used for cooking and lighting purposes in many parts of Asia and Africa where it is sold in beverage bottles and jerry cans in both commercial and residential places b...
Genetic Variability in Cultured and Wild Population of Clarias gariepinus Using Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE)
Aim: The present study was undertaken to determine the genetic differences between cultured and wild populations of Clarias gariepinus in Southwest Nigeria using Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis...
The Effect of Colchicine on Adventitious Shoot Regeneration from Cultured Leaf Explants of Petunia hybrida
Aims: The effect of various concentrations of colchicine on adventitious shoot regeneration of two Petunia hybrid cultivars was studied. Study Design: All collected data were analyzed using the SAS software. One-way anal...
Identification of Biomarkers of Stress in Meat of Pigs Managed under Different Mixing Treatments
Aims: The objective of this work was to apply the proteomic approach for identification of animal-based stress biomarkers during pig muscle maturation. Study Design: Pigs were subjected to management treatments that may...