Effect of Auxin and Cytokinin on Regeneration, Total Phenolics and In vitro Antioxidant Activities of Bacopa monnieri (L.) Pennell
Journal Title: Journal of Ayurvedic and Herbal Medicine - Year 2019, Vol 5, Issue 2
Abstract
Context: Cells are protected from cellular harm by antioxidant compounds. These compounds secure cells from h Cells are protected from cellular harm by antioxidant compounds. These compounds secure cells from harmful free radicals which initiated oxidative stretch. Bacopa monnieri (L.) Pennell (Schropulariaceae) have medicinal properties, such as sedative, tranquilizing, antioxidant and memory-enhancing effects. Objective: To determine the total phenolic content and concentrations of flavonoids and also in vitro antioxidant activity in methanol extract of shoot culture from leaves explants of Bacopa monnieri (L.) Pennell supplemented with various combinations of auxin and cytokinin hormone. Material and method: Murashige and Skoog (MS) media was selected for the experiment and different concentration of auxin and cytokinin were added in media. Leaves explants were inoculated on media for callus initiation. Indirect shoot formation occurred on calli of leaf explants after subculture. Total phenolic, flavonoid content and in vitro antioxidant activities such as DPPH radical scavenging activity, hydrogen peroxide (H2O2) scavenging activity and ferric reducing power of the shoot cultures are quantitatively estimated by UV spectroscopy. Result: The highest content of total phenolics (3.14g/100g d.w.) and flavonoids (0.565 g/100g d.w.) were obtained in shoots regenerated on the MS medium supplemented with BA (2mg/lit) and IAA (1mg/lit). Shoot culture on MS medium supplemented with BA (2mg/lit) and IAA (1mg/lit) showed the highest DPPH radical scavenging activity (85.31%), highest H2O2 scavenging activity (75.31%) and highest ferric reducing power (25.3µg/g). Discussion and conclusion: Excellent in vitro antioxidant activity was given by extract of Shoot culture on MS medium supplemented with BA (2mg/lit) and IAA (1mg/lit). So they can be used as natural antioxidants after isolation and purification.
Authors and Affiliations
Aziz Ahmed, Sayeed Ahmad, Mohd Shuaib, Rizwan Ahmad, Neelam Somani, Ankita Sharma
Keywords
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