Effect of blue light and white light on proliferation of ARPE-19 cells detected by CCK-8
Journal Title: Guoji Yanke Zazhi - Year 2018, Vol 18, Issue 8
Abstract
AIM: To investigate the effects of blue light and white light on the proliferation of human retinal pigment epithelial cell line(ARPE-19)at different times, and lay a foundation for further detecting the changes of related factors during photodamage and further studying the signal transduction mechanism during light damage. <p>METHODS:Well-grown ARPE-19 cells were collected for experimentation. The standard curve of CCK-8 was made to determine the proper cell density of ARPE-19 cells and the reaction time of CCK-8 reagent. The cells were divided into dark group, blue light group and white light group, which were irradiated for 3, 6 and 9h respectively. After 12h of light-repellent treatment, CCK-8 method was used to examine the effects of different light sources on the proliferation rate of ARPE-19 cells at different times. <p>RESULTS: The number of cells per well was selected by CCK-8 standard curve to be 20 000, and the corresponding absorbance value was measured after 4h of the action of CCK-8 solution. The CCK-8 test results showed that the cell proliferation rates of the three groups were significantly different(<i>P</i><0.01). The cell proliferation rate of the blue light group was significantly different(<i>P</i><0.001)at 3, 6 and 9h, and the cell proliferation rate decreased gradually with the extension of the illumination time. The cell proliferation rate of the white light group was significantly different(<i>P</i><0.05)at 3, 6 and 9h; there was a statistically significant difference in the rate of cell proliferation at 3h and 6h in white light(<i>P</i><0.05), however, there was no significant difference in the rate of cell proliferation at 9h illumination compared with 3h and 6h illumination respectively(<i>P</i>=0.253, 0.120). The proliferation rate of cells under white light for 3-6h showed a downward trend, while that of cells under light for 6-9h showed an upward trend. At the same illumination time, the proliferation rate of the cells in the blue and white groups was lower than that in the dark group, and the cell proliferation rate in the blue group was lower than that in the white group. The difference was statistically significant(<i>P</i><0.05). <p>CONCLUSION: The proliferation of ARPE-19 cells was inhibited by light irradiation. The proliferation rate of cells in blue light group was significantly lower than that of white light group. With the increase of light time, the cell proliferation rate decreased.
Authors and Affiliations
Shen - Wen Liu
Keywords
Related Articles
- EP ID EP547941
- DOI 10.3980/j.issn.1672-5123.2018.8.06
- Views 110
- Downloads 0
Change of macular microperimetry in diabetes patients without diabetic retinopathy
AIM: To investigate the macular function of patients with diabetes mellitus(DM)who had no diabetic retinopathy(DR). <p>METHODS: Totally 140 eyes of 70 subjects with diabetes who had no vision disorders and fundus abnorma...
Recent progress of formation mechanisms and factors related to postoperative visual function in patients with IMEM
Idiopathic macular epiretinal membrane(IMEM)is a common macular area disease associated with age and characterized by symptoms of decreased visual acuity and metamorphopsia. With the acceleration of aging population, the...
Effect and safety evaluation of Qiming Granule on the treatment of nonproliferative diabetic retinopathy
AIM:To explore the therapeutic effect and safety evaluation of Qiming Granule on non-proliferative diabetic retinopathy(NPDR). <p>METHODS: Totally 94 cases(120 eyes)of NPDR patients treated in endocrinology department of...
Use of the lower concentration of trypan blue staining technique during cataract surgery
AIM: To determine the lowest concentration of trypan blue(TB)used to stain the anterior capsule satisfactorily and to evaluate the clinical significance of trypan-blue-assisted capsulorhexis in cataract surgery. <p>METHO...
Combined treatment of Conbercept and panretinal photocoagulation for neovascular glaucoma after central retinal vein occlusion
AIM: To investigate the efficacy of the combined treatment of Conbercept and panretinal photocoagulation(PRP)for neovascular glaucoma(NVG)with central retinal vein occlusion(CRVO). <p>METHODS: The clinical data of 100 NV...