Effect of some chemicals inducers on terpenes production (ginkgolide A and bilobalide) in callus of Ginkgo bilobaL. plant
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2017, Vol 12, Issue 4
Abstract
The present experiment was carried out to study the effectof some inducers i.e. casein (0.5, 1, 1.5 and 2 g), gibberellic acid(0.1, 0.5,1 and 2 ppm) and sucrose(45,60, 75 and 90 g) on callus growth, terpenesproduction (ginkgolide A and bilobalide) and some biochemical constituents in Ginkgo biloba L. callus. Data showed that the highest callus induction was shown on Murashige and Skoog (MS) medium supplemented with 1.0 mg/l of NAA and 1.0 mg/l kinetin. The highest amount of ginkgolideA(0.269 mg/g fresh weight) was recorded at rate 0.5 g of casein. While, highest concentration of bilobalide (4.00 mg/g) was obtained with75 g/l sucrose. In the same direction, the maximum value of callus weight was associated with the highest concentration of gibberellic acid (2 ppm) compared with the control. On the contrary, the lowest fresh weight is obtained by the addition of the high concentration of sucrose 90 g/l. While, the maximum value of malondialdehyde and hydrogen peroxide are associated with the highest concentration of sucrose. In conclusion, Ginkgo bilobaL.callus cultured showed capacity for producing ginkgolide A and bilobalide and could be an alternative renewable natural source for its large scale production. Moreover, some of inducers may contribute to increase active constituents with reduced the cost production in callus culture of Ginkgo biloba L.
Authors and Affiliations
Abd El-monemM. Sharaf1, MohamedH. Hendawey2 ,, Mohamed I. Diab, HamdyM. Ibrahim2
Keywords
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- EP ID EP390050
- DOI 10.9790/3008-1204040818
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