Effect of some chemicals inducers on terpenes production (ginkgolide A and bilobalide) in callus of Ginkgo bilobaL. plant
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2017, Vol 12, Issue 4
The present experiment was carried out to study the effectof some inducers i.e. casein (0.5, 1, 1.5 and 2 g), gibberellic acid(0.1, 0.5,1 and 2 ppm) and sucrose(45,60, 75 and 90 g) on callus growth, terpenesproduction (ginkgolide A and bilobalide) and some biochemical constituents in Ginkgo biloba L. callus. Data showed that the highest callus induction was shown on Murashige and Skoog (MS) medium supplemented with 1.0 mg/l of NAA and 1.0 mg/l kinetin. The highest amount of ginkgolideA(0.269 mg/g fresh weight) was recorded at rate 0.5 g of casein. While, highest concentration of bilobalide (4.00 mg/g) was obtained with75 g/l sucrose. In the same direction, the maximum value of callus weight was associated with the highest concentration of gibberellic acid (2 ppm) compared with the control. On the contrary, the lowest fresh weight is obtained by the addition of the high concentration of sucrose 90 g/l. While, the maximum value of malondialdehyde and hydrogen peroxide are associated with the highest concentration of sucrose. In conclusion, Ginkgo bilobaL.callus cultured showed capacity for producing ginkgolide A and bilobalide and could be an alternative renewable natural source for its large scale production. Moreover, some of inducers may contribute to increase active constituents with reduced the cost production in callus culture of Ginkgo biloba L.
Authors and Affiliations
Abd El-monemM. Sharaf1, MohamedH. Hendawey2 ,, Mohamed I. Diab, HamdyM. Ibrahim2
Self-medication of antibiotics is becoming a common type of self-care behavior among the population of many countries. Unwanted use and vague use of antibiotics have led to a bigger problem, which is drug resistance. A l...
Biosynthesis and characterisation of zinc doped iron oxide nanoparticles from pedalium murex and its new avenues in pharmacological applications
Biosynthesis of metal oxide nanoparticles gains attention over the past decades due to its ecofriendly nature and provides nanoparticles with controlled size and shape. In the present work, zinc doped iron oxide nanopart...
The viruses of Hepatitis B and C (HBV & HCV) usually cause chronic infection which may develop the Cirrhosis and Hepatocellular carcinoma (HCC). Moreover, carriers of chronic hepatitis become the source of transmission o...
To study the medication adherence among type 2 DM patients at a tertiary care hospital. Materials and methods: A cross sectional observational study was conducted for a period of 6 months in a general medicine department...
Gross Anatomical, Histological and Histochemical Studies of Thyroid Glands of African Giant Rat (Cricetomys gambianusWaterhouse, 1840)
The wild African giant rat is currently explored as a laboratory model for scientific research. The present study is aimed at investigating the gross morphology, histology and histochemistry of the thyroid gland of wild...
How To Cite
Abd El-monemM. Sharaf1, MohamedH. Hendawey2, , Mohamed I. Diab, HamdyM. Ibrahim2 (2017). Effect of some chemicals inducers on terpenes production (ginkgolide A and bilobalide) in callus of Ginkgo bilobaL. plant. IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS), 12(4), 8-18. https://europub.co.uk/articles/-A-390050