Impact of ultrasound treatment on molecular structures of casein
Journal Title: JOURNAL OF ADVANCES IN BIOTECHNOLOGY - Year 2012, Vol 2, Issue 2
Abstract
Casein was modified by ultrasound treatment (160W, 400 W)and the structural characteristics of casein (control), USC-1 (160W of ultrasound treated casein) and USC-2 (400W of ultrasound treated casein) were investigated. Ultrasound treatment resulted in increase in the numbers of protein bands of casein in region of low molecular weight according to SDS-PAGE pattern. The relative percentage of the proteins with molecular mass (MM) of over 30 kD in USC-1 and USC-2 significantly decreased compared with control sample. Coupled with this is the relative percentage of the proteins with MM of region 20 kD-30 kD for the ultrasonic-treated caseins increased. Contents of α-helix, β-sheet and random coil of casein for USC-1 and USC-2 decreased compared with control. While, β-turn content for USC-1 and USC-2 increased. Ratio of α-helix to β-sheet in USC-1 and USC-2 significantly decreased (from 0.70 to 0.47 and 0.46). Ultrasound treatment had a strong crushing action of surface of the casein micelle and resulted in its structural disruption. Particle size distribution range of casein was approximately 0.5-70 μm, Particle size of USC-1 and USC-2 was less than 30μm. D (50), D (4, 3) as well as D (3, 2) for USC-1 and USC-2 reduced compared with the control. The ratio of D (4, 3) to D (3, 2) also decreased with increase of ultrasonic intensity. This study demonstrated that ultrasound treatment could be an effective method for modification of casein structure. This modification is the basis on improvement in functional properties.
Authors and Affiliations
Jinshui Wang, Yanli Yin, Feng Jia
Keywords
Related Articles
- EP ID EP653823
- DOI 10.24297/jbt.v2i2.1702
- Views 79
- Downloads 0
AN INTEGRATIVE TAXONOMY OF MOLECULAR AND TRADITIONAL APPROACHES FOR IDENTIFICATION OF A STORAGE MITE, ALEUROGLYPHUS OVATUS (ACARI: ASTIGMATA: ACARIDAE) IN MALAYSIA
A reliable and rapid taxonomic identification of a mite is the basis for a correct diagnosis of important mite associated allergies as they produce species-specific allergens. A double approach (molecular and morphologic...
Effects of n-butanol fraction of Gongronema latifolium leave extract on some kidney function and histological parameters in CCl4-induced oxidative damage in Wistar albino rats.
Effects of n-butanol fraction of Gongronema latifolium leave extract on some kidney function and histological parameters in (CCl4) carbon tetrachloride-induced oxidative damage in Wistar albino rats were assessed. Fifty-...
Comparative Proteomics Study of Outer Membrane Proteins from three Pathogenic Leptospira Species used in Vaccine
Background and Objective: Outer Membrane Proteins (OMPs) have an important role in pathogenecity and immunogenecity of Leptospira introgans. The aim of this study was chemical and immunological analysis of OMP extracted...
Secondary multiplication of somatic embryos in banana (Musa spp. AAA) in semisolid medium: effect of the culture vessel type and sterilization method
The objective of this work was to evaluate the influence of the type of culture vessel, and sterilization method on secondary multiplication of somatic embryos of the banana cultivar Grande naine (AAA) in semisolid cul...
Effects of Different Paclobutrazol-Cultar Concentrations on the Micropropagation of Sugarcane (Saccharum Officinarum) Variety CPCL99-4455
Sugarcane (Saccharum officinarum), being an important cash crop in Belize, accounts for 60% of agricultural exports providing employment for thousands of Belizeans. This research was carried out to determine the effects...