IN VITRO ANTIMICROBIAL AND ANTIOXIDANT ACTIVITY OF GINKGO BILOBA BARK EXTRACT
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2012, Vol 3, Issue 6
Abstract
Ginkgo biloba also called as Maidenhair Tree, belongs to the family Ginkgoaceae. In this study, the hydro-Methenolic extract from bark of Ginkgo biloba was screened for their in vitro antimicrobial and antioxidant activity. The antibacterial activities of extract against both Gram-positive & Gram-negative bacteria was determined by agar well diffusion method, minimum inhibitory concentration (MIC), relative percentage inhibition and antioxidant activity was determined by means of 1,1-diphenyl-2-picryl hydrazyl (DPPH), Hydrogen peroxide (H2O2) and reducing power scavenging activity. The antimicrobial potency of extract based on their zones of inhibition (mm) and MIC values and relative percentage inhibition.The extract exhibited maximum zone of inhibition against Streptococcus faecalis and minimum zone of inhibition against Escherichia Coli. The MIC values of extract and standard drug ranges between 2.5-6 mg/ml and 0.92-3.5 μg/ml respectively. Extract exhibited maximum relative percentage inhibition against Streptococcus faecalis (77.4 %) and lowest against Proteus vulgaris (42.03 %). For antioxidant activity, results of 1,1-diphenyl-2-picryl hydrazyl and hydrogen peroxide method, was expressed as IC50. Ascorbic acid was used as standard showed IC50 of 20.4 μg/ml & 95 μg/ml respectively whereas extract of bark of Ginkgo biloba showed IC50 of 40 μg/ml & 105 μg/ml respectively. In reducing power method the reducing power of extract was determined and compared with standard ascorbic acid, increased absorbance with increased concentration significantly increase in reducing power. The extract showed the potent antioxidant and antimicrobial activity, which concludes that the extract can be used as antimicrobial and antioxidant agent.
Authors and Affiliations
Aman Kaur , Parminder Nain , Jaspreet Nain
Keywords
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