In vitro multiplication of ‘FHIA-25’ (Musa spp., AAB) in Systems Temporary Immersion
Journal Title: Biotecnología Vegetal - Year 2011, Vol 11, Issue 1
Abstract
Necessity to produce high quality planting material has required searching new alternatives to increase the efficiency of in vitro propagation methods and their automation, as in Temporary Immersion Systems (TIS). This work was aimed to multiply the hybrid ‘FHIA-25’ (Musa spp. AAB) in TIS. The effect of different culture medium volumes per explant and densities of planting materials per culture flask at the same immersion frequency was determined. These two factors showed influence on the evaluated variables. A volume of 40 ml of culture medium per explant and densities of 80 explants per flask were selected to multiply this cultivar in TIS. These results permitted to increase in vitro production of high-quality plants for rooting stage.
Authors and Affiliations
Milagros Basail Pérez , Victor Medero Vega, Eneida Otero Gálvez, Marlenys Torres Delgado, Manuel Cabrera Jova, Jorge López Torres, Arletys Santos Pino, Aymé Rayas Cabrera, Maricel Bauta Toledo, Eriker Páz Chávez, Yoel Beovidez García, Alexis Ortega Ortiz, José Enrique Pérez Martínez
Keywords
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