L-cysteine inhibits LPS-induced microglial overactivation and autophagy
Journal Title: Journal of Air Force Medical University - Year 2023, Vol 44, Issue 5
Abstract
Objective To clarify the effects of L-cysteine (L-Cys) on lipopolysaccharide (LPS)-induced inflammatory reaction and autophagy in microglia and to explore its mechanism. Methods LPS-activated BV2 microglia were preprotected with L-Cys. Cell viability was detected by CCK-8 assay and its morphology was observed by immunofluorescence staining. In vitro scratch assay was used to detect cell migration ability. Western blotting and immunofluorescence staining were used to detect the expression of autophagy-related proteins and the changes of P38 MAPK / ERK1 / 2 signaling pathway in BV2 microglia. Results Compared with LPS group, L-Cys pre-protection could: ①increase the cell viability of BV2 microglia (P < 0. 01); ② inhibit the cell activation of BV2 microglia ( P < 0. 05);③ inhibit the cell migration of BV2 microglia (P < 0. 01); ④inhibit the excessive autophagy of BV2 microglia (P < 0. 01 or P < 0. 05); ⑤decrease the protein expression level of P38 MAPK / ERK1 / 2 signaling pathway (P < 0. 01 or P < 0. 05). Conclusion L-Cys pre-conditioning can inhibit LPS-induced overactivation of BV2 microglia and inhibit LPS-induced hyper-autophagy in BV2 microglia through P38 MAPK / ERK1 / 2 signaling pathway.
Authors and Affiliations
QI Jingyu, SONG Dake, GUI Jiayue, YUE Jiao, WANG Xinshang, LIU Shuibing, ZHANG Kun
Keywords
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