Localisation of the gap junctions in the trigeminal ganglia of the rat using various techniques
Journal Title: INTERNATIONAL JOURNAL OF RECENT TRENDS IN SCIENCE AND TECHNOLOGY - Year 2017, Vol 25, Issue 1
Abstract
Background: Gap junctions form the important connecting channels among the neurons and the surrounding cells. Various neurotransmitters pass through them. Gap junctions were made up of Connexons. These connexon subunits of two cells joined with each other to form the gap junctions. In the present study, localization of gap junctions was done in trigeminal ganglia using various techniques. This ganglion consists of neurons of varied sizes, which were surrounded by satellite glial cells. Satellite glial cells from the envelope like structure around the glial cells and in turn responsible for controlling the internal homeostasis of the neuron. Increase activity of these cells were seen in many of the pathological conditions such as trigeminal neuralgia Material Methods: The present study was done on the adult male wistar rats (n=18). These rats were randomly divided into three groups, depending upon the procedure performed. Group I (n=6) was utilized for the cresyl violet staining to identify various sizes of neurons, group II (n=6) had undergone immunohistochemistry for the connexin-43 antibody and the group III, electron microscopic study was done to identify and confirm the location of gap junctions Results: In the present study, trigeminal ganglia from both the left and right sides were examined for morphological, immunohistochemical and electron microscopic localization of gap junctions. Morphological studies showed that ganglia were composed of neurons surrounded by satellite cells, nerve fibers along with Schwann cells and connective tissue containing blood vessel. Immunohistochemistry showed prominent gap junctions between the neurons and satellite glial cells of the ganglia. Electron microscopy revealed the presence of gap junctions in the trigeminal ganglion. No difference was noted between the trigeminal ganglia of the left and right sides. Conclusion: Gap junctions forms the important channels for the transmission of various neurotransmitters. Cresyl violet staining did identification of cells in the trigeminal ganglion. After identification, further confirmation was done by the immunohistochemistry and electronmicrosopic study. Localization of the gap junctions is important to identify the sites for exchange and in future these sites can be utilized for various gap junction blocker studies.
Authors and Affiliations
Sanjay Kumar Sharma, Vishwajit Ravindra Deshmukh, Pranav Prasoon, Subrata Basu Ray
Keywords
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- EP ID EP493850
- DOI 10.26611/202511
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