MASS PRODUCTION OF THE BENEFICIAL NEMATODE Heterorhabditis bacteriophora ON SOLID MEDIA USING SOLID STATE FERMENTATION TECHNOLOGY
Journal Title: International Journal of Agriculture Sciences - Year 2016, Vol 8, Issue 55
Abstract
The focus of this study was to mass produce the entomopathogenic nematode (EPN), Heterorhabditis bacteriophora as a bio-control agent (bio-pesticide) using its symbiont bacteria Photorhabdus luminescens on a solid media surface. The process of growing these nematodes was to upscale the surface area of a solid agar media, thus increasing the yield of the beneficial nematodes. The solid agar media was adjusted to conditions, which provided an ideal growing environment for these nematodes to maintain vitality for an entire life cycle. The bacterial symbiont was then inoculated by an in-vitro culture 24 hours prior to nematode inoculation and furthermore leading to the inoculation of Heterorhabditis bacteriophora. The inoculated entomopathogenic nematodes have a 7-8 day life cycle .Once the nematodes develop into infective juveniles, the hermaphrodites can begin to self-fertilize its eggs and produce new offspring. Numbers of offspring then maximize after approximately seven days post-nematode inoculation. After harvesting, the nematodes are sanitized and stored for further use. The yield of H. bacteriophora was 5.5x105 IJs/gram with variation of 10%. The scale-up technology used in this study can be further improved by altering solid media and to optimize the growth conditions using larger surface area.
Authors and Affiliations
DEVANG UPADHYAY
Keywords
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