Micropropagation of Plumbago zeylanica L. using in vitro germinated seedling explants
Journal Title: International Journal of Life Science - Year 2017, Vol 5, Issue 2
Abstract
In vitro seed germination in Plumbago zeylanica L. has been achieved using combined treatment of seed storage, seed soaking, mechanical scarification and MS-basal medium supplemented with GA3. P. zeylanica seeds stored for six months soaked in distilled water for 30h showed maximum seed germination (95.55%) on ½–Murashige and Skoog basal+GA3 (3.61 µM). Hardening and survival of seedlings obtained from seed germination was 86%. Maximum multiple shoots/explant (18.02±0.09) proliferated from nodal explants cultured on MS-basal medium supplemented with BAP, Kn and Zea (4.44+4.56+2.32 µM). Maximum shoots rooted on ½–MS basal+IBA (6.15 µM) having 21.91±1.44 roots/shoot and root length 9.36±0.56 cm. Successful hardening of plantlets showed 94% of survival after transferring to garden condition. To the best of our knowledge, this is the first report where in vitro developed seedling has been used as an explant source which will nullify the level of contamination in cultures. We have successfully established an efficient protocol for in vitro seed germination, multiple shoot production through nodal culture and rooting of regenerated shoots to achieve an optimized plant regeneration system along with acclimatization in natural condition. This protocol can be used for large scale commercial micropropagation and conservation purpose.
Authors and Affiliations
Prachi Sharad Kakade, Nutan P Malpathak
Keywords
Related Articles
- EP ID EP542447
- DOI -
- Views 78
- Downloads 0
Studies on physico-chemical parameters of Mul Lake, Maharashtra, India
India has 2.4 % of worlds land mass 4 % as water resources and 16 % of worlds population therefore no scope to underutilize water resources. Since the quality of the aquatic life depends on water quality, a thorough asse...
Folklore medicine of primitive tribals in Dumbriguda Mandal, Visakhapatnam District, Andhra Pradesh, India
Folklore medicine survey was conducted among the primitive tribal community residing in Dumbriguda Mandal, Visakhapatnam district, Andhra Pradesh. The prime objective of the study is to document the traditional medicinal...
The community structure of demersal fish species from bottom-trawls off Namibia and the West coast of South Africa
Changes in the structure and composition of the demersal fish assemblages are assessed using a variety of ecosystem indicators known to capture such changes, which may be induced by bottom-trawling. The aim of this study...
Encapsulation of Lactic acid bacteria in calcium alginate beads for higher bacteriocin production
Lactic acid bacteria (LAB) strains LAB -A, LAB -B and LAB-C isolated from batter of idli, very popular fermented food of south India and identified to species level using 16 S rRNA sequencing as Pediococcus acidilactici...
Quality of life evaluation in women Psoriatic patients
Background: Psoriasis is a common chronic, recurrent, immune mediated disease of the skin and joints, found worldwide but the prevalence varies among different ethnic groups. It is associated with significant physical an...