Micropropagation of the Cuban Papaya Hybrid IBP 42-99
Journal Title: Biotecnología Vegetal - Year 2002, Vol 2, Issue 4
Abstract
This work consisted of developing a methodology to propagate papaya hybrids through in vitro culture. As plant material, lateral buds of plants of the hybrid IBP 42-99 were used. The explants were established following the methodology proposed by Posada et al (1999). For the multiplication stage, the combinations of 6-BAP (0.225, 0.45 and 0.90 mg.l-1) and ANA (0.14 and 0.28 mg.l-1) were studied. In the rooting stage, a comparison was done between the culture media proposed by Drew (1988) and Posada (1995). The culture medium containing MS salts (1962) supplemented with 0.45 mg.l-1 of 6-BAP and 0.14 mg.l-1 of ANA was the most adequate for multiplication, while the best medium for rooting was 5mg.l-1 of AIB with ten days in the culture medium, where 68% of rooted plants was obtained and in the acclimatization phase 71% of plant adaptation was achieved.
Authors and Affiliations
Jorge Gallardo Colina, Laisyn Posada-Pérez, Rafael Gómez-Kosky, Leticia Más Castellanos, Maritza Reyes, Idalia Herrera Ofarri
Keywords
Related Articles
- EP ID EP697822
- DOI -
- Views 71
- Downloads 0
Effect of phenolization on sugarcane explants (Saccharum spp. Hybrid var. Sp 70-1284) on callus formation
Sugarcane (Saccharum spp. hybrid) is the crop were more work has been done on biotechnological aspects because of its economical importance among the comercial crops. When the explants from the field of the hibrid SP 70-...
Selection of guava clonal lines of the cultivar Enana roja (EEA 18-40) for use in genetic improvement and propagation
The present work was carried out with the objective of evaluating and selecting in field conditions clonal lines of guava (variety Enana Roja, EEE 18-40) according to the fruits characters. The plants used were obtained...
Effect of inoculum density and gaseous atmosphere renovation in Digitalis purpurea L. shoots culture in Temporary Immersion Systems
Digitalis purpurea L. is one of the two unique species of economic interest in the genus Digitalis. It is important because is one of the principal source of cardenolides, irreplaceable drugs in the treatment of heart f...
Genetic transformation by Agrobacterium tumefaciens in papaya hybrid from in vitro plant shoot-tips
In papaya (Carica papaya L.) viral infections can decrease production by more than 50%. Genetic transformation consists of an alternative means of obtaining new varieties, which are resistant to this pathogen. The main...
Optimal dose of Gamma radiation for Phaseolus vulgaris L. cultivar ‘BAT- 93’ in vitro plants regeneration
The application of Gamma radiations combined with tissues cultures in common bean constitutes a tool to accelerate the programs of genetic improvement. The determination of the optimal radiation dose is an important as...