ISOLATION, IDENTIFICATION AND CHARACTERIZATION OF POLYHYDROXYBUTYRATE PRODUCING Bacillus flexus
Journal Title: International Journal of Engineering Sciences & Research Technology - Year 30, Vol 5, Issue 11
Abstract
Dwindling crude oil reservoirs and increasing environmental and societal concerns over harmful effects of synthetic plastics have increased the pressure on development of sustainable and environmentally friendly biopolymers like Polyhydroxybutyrate (PHB). Present work aimed at isolating and characterizing PHB producing bacteria from soil. Isolation from rhizospheric soil samples yielded 85 isolates, which were screened for PHB production by Sudan Black B followed by Nile Blue A staining procedures. Overall, 21.18% isolates tested positive for PHB production. The polymer production by the isolates was found to vary from 0.011- 0.186 g/L of the dry cell weight. The highest PHB yield was observed in R8, which was characterized for its morphological and biochemical properties. Based on its 16S rRNA gene sequences, isolate was identified as Bacillus flexus. The ability of the organism to produce protease, amylase and lipase indicated possibility of using various low cost agro-industrial, food and dairy wastes as carbon substrates for cost-effective PHB production. Its hydrolytic potential coupled with resistance to heavy metals and antibiotics can be explored for PHB production using waste water.
Authors and Affiliations
Pradnya Damle
Keywords
Related Articles
- EP ID EP128703
- DOI 10.5281/zenodo.164908
- Views 71
- Downloads 0
Malicious Code Analysis using Metalanguage
Computer viruses are big threat to computer world; researchers doing work in this area have made various efforts in the direction of classification and detection methods of these viruses. Graph mining, system call...
Analysis of Regenerator for Reversed Stirling Cycle Which Various Geometry
Analysis of regenerator deals with assumptions made for analysis and different equation use to predict the performance of regenerator Problem and sample calculation deals with problem and calculation of various paramete...
Rheological Study of Petroleum Fluid and Oil-in-Water Emulsion
The rheological behavior of two types of crude oil and oil-in-water (o/w) emulsion were thoroughly studied. These rheological properties were investigated including steady flow behavior, shear stress, shear rate,...
Review Study of Natural Convection Heat Transfer on Heated Plate by Different Types of Fin Array
Fins are also called as extended surfaces the main purpose of that is to increase the heat transfer rate Fins offer an economical and trouble free solution in many situations demanding natural convection heat tran...
Comparative Study of Surface Morphology and Spectroscopic Analysis for Corona and Chromic Acid Treated Polyethylene Film for Water Based Ink Printing
Polyethylene, Specially the LDPE is one of the most widely used thermoplastic in packaging industries, due to its low cost, broad range of properties like excellent moisture barrier and chemical resistance which a...