Patogenicity of free-living amoebas isolates in body waters from Lima

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Patogenicity of free-living amoebas isolates in body waters from Lima

Journal Title: Revista Peruana de Medicina Experimental y Salud Publica - Year 2008, Vol 25, Issue 2

Abstract

[b][i]Objectives. [/i][/b]To determine the presence of free-living amoeba (FLA) in water bodies from Lima department and assess their pathogenic ability in normal mice and immunosuppressed. [b][i]Material and methods.[/i][/b] Water samples were collected from rivers, lakes, swimming pools and wells in Lima, Peru. The isolation was performed by culture in non-nutritious agar with 2% of [i]E. coli [/i]or [i]E. aerogenes[/i] at 37 °C. Subsequently were injected into normal mice and immunosuppressed (betamethasone 1.4 mg/mL, a single dose) with a suspension of amoeba isolated. For the 15 days were assessed histological damage in both groups. [b][i]Results.[/i][/b] AVL were identified in 40/83 samples, 31.3% of the samples were developed in cultures. 26 strains were isolated from AVL of seven genera (Hartmannella, Acanthamoeba, Mayorella, Naegleria, Vahlkampfia, Vannella and Saccamoeba), Acanthamoeba was the most frequent (44.2%). AVL was found in 53.8% of immunosuppressed mice and 15.4% of the normal (p <0.05). [b][i]Conclusions.[/i][/b] AVL exist in water bodies from Lima, which have a potential pathogen in immunosuppressed patients, there is a risk of amoebic infection associated with these water sources.

Authors and Affiliations

María del Carmen Garaycochea , María Beltrán, Cecilia Morón

Keywords

EP ID EP107640
DOI -
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