Phytochemical and Antimicrobial Screening of Root Extracts of Dacryodes edulis
Journal Title: Biotechnology Journal International - Year 2017, Vol 19, Issue 3
Abstract
Aims: To screen extracts of Dacryodes edulis phytochemically. To assay the said extracts for antimicrobial activity. To validate or otherwise claims by traditional medicine practitioners on Dacryodes edulis. Place and Duration of Study: Department of chemistry, University of Agriculture, Makurdi, Nigeria, from October 2016 to November 2016. Methodology: Roots of Dacryodes edulis extracted using n-hexane, ethyl acetate, and methanol, were phytochemically screened for presence of secondary metabolites. Anti-microbial assay of extracts was done using Methicillin resist Staph. aureus, vancomycin resist enterococci, Staphylococcus aureus, Escherichia coli, Salmonella typhi, Proteus mirabilis, Pseudomonas aeruginosa, Candida albicans, Candida krusei, and Candida tropicalis. Results: Results showed presence of steroid/triterpenes, phenols, reducing sugars, cardiac glycosides, alkaloids and flavonoids. Anti-microbial screening of extracts showed sensitivity against six microbes: Staphylococcus aureus; Salmonella typhi; Pseudomonas aeruginosa, methicillin resist, staphylococcus aureus, Candida albicans and Candida krusei. Ethyl acetate extract exhibited highest zones of inhibition against Staphylococcus aureus and Candida albicans (28 mm) and highest growth inhibitory effects (MIC) for the microbes at concentrations of 1.25 mg/mL and 2.5 mg/mL. All other extracts had Minimum Bactericidal/ Fungicidal Concentration (MBC/MFC), values of 2.5 mg/mL, 5 mg/mL and 10 mg/mL respectively. Conclusion: This study has justified the traditional use of the plant for treatment of various diseases that are caused by these organisms.
Authors and Affiliations
P. J. Nna, T. A. Tor-Anyiin, J. O. Igoli, M. E. Khan, J. V. Anyam
Keywords
Related Articles
- EP ID EP315057
- DOI 10.9734/BJI/2017/35311
- Views 137
- Downloads 0
Morphological Anomalies in Somatic Embryo Structure in Catharanthus roseus: Improving Embryo Germination by Amending Plant Growth Regulators, Activated Charcoal and Sucrose Level
The present study describes the incidence of somatic embryo (SE) irregularities in Catharanthus roseus (L.) G. Don. In this ornamental and anti-cancerous medicinal plant, SEs were developed at a very high rate from hypoc...
Determination of Starch and Cyanide Contents of Different Species of Fresh Cassava Tuber in Abia State, Nigeria
This investigation aimed at determining the cyanide and starch contents of different species of cassava grown in Abia State. Samples of cassava tubers were purchased from the local market and identified by a taxonomist f...
Characterization of Bacteria Community Isolated from Wood Decay
In order to discover enzymes having potential for wood fibre modification, bacteria (fourteen strains designated MMB1 to MMB14) were isolated from a decomposing stump from a resinous tree. Phylogenetic analysis and bioch...
Synthesis, Characterisation, Antibacterial and Cytotoxic Assay of Zinc Oxide (ZnO) Nanoparticles
Aims: The objective of the present experiment was to synthesise ZnO nanoparticles by the environmental friendly process of bioreduction of ZnO salt with Camellia sinensis crop shoot extract and post synthesis to characte...
Genetic Improvement of Saccharomyces boulardii R7 and Generate Suitable Strains for Synthesis and Expression of Recombinant Products
Saccharomyces boulardii R7 was mutagenized using UV radiation. Uracil auxotroph mutants were isolated and four of these mutants showed stable mutation. Two mutants SbR7M7 and SbR7M10 were chose for transformation accordi...