PHYTOCHEMICAL SCREENING OF SECONDARY METABOLITES FROM MYXOPYRUM SERRATULUM A.W. HILLS BY GC-MS, HPTLC ANALYSIS
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2016, Vol 7, Issue 6
Abstract
Myxopyrum serratulum is a valuable medicinal plant widely distributed in Western Ghats at the altitude of 600-900m. Traditionally, the leaves of the plant used to treat asthma, cough diabetes and nerve complaints. The current study have been investigated the presence of various secondary metabolites such as saponin, flavonoids and terpenoids in the methanolic extract of the plant by Thin layer Chromatography (TLC) using appropriate solvent system and the quantification of secondary metabolites like total saponin and terpenoid content was performed by gravimetric analysis. The aqueous fraction of M.serratulum was characterized using GC-MS analysis and presence of iridoid glycoside in the ethyl acetate,n-butanol fractions and methanolic extract was identified by HPTLC analysis. Total terpenoid and saponin content have been observed as 6.4± 0.33, 3.815± 0.56 respectively. Phytochemical investigation indicated that the presence of flavonoids, Phenols and higher concentration of tannin, saponin in this plant.
Authors and Affiliations
Vijayalakshmi Maruthamuthu, Ruckmani Kandasamy
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