Prevalence of virulence factors and antifungal susceptibility testing of Candida species isolated from clinical specimens
Journal Title: Indian Journal of Microbiology Research - Year 2018, Vol 5, Issue 1
Abstract
Introduction The main aim of our study is to isolate and identify Candida species from clinical samples and to detect the virulence markers and also to determine their susceptibilities to various antifungal agentsMaterials and Methods Eighty Candida isolates from respiratory samples were included in the study Detection of various virulence markers of candida spp such as Phospholipase Proteinase Hemolysin and Biofilm production was carried out by Phenotypic methods Antifungal susceptibility testing of the isolated yeasts for Amphotericin B Itraconazole Fluconazole Caspofungin and Voriconazole was determined by Etest methodsResults In our study most common spp isolated was Candida albicans65 followed by Candida tropicalis15 and Candida glabrata10Prevalence percentage of virulence factors such as Hemolysin Proteinase Biofilm and Phospholipase production in Candida spp were 85 775 688 and 45 respectively The susceptibility pattern of Candida isolates to antifungal agents showed highest resistant rate of 338 for Itraconazole and lowest resistant rate of 25 for CaspofunginConclusion Presence of virulence factors in Candida species might indicate invasiveness and its relation with infection Hence the significance of virulence determination and antifungal susceptibility testing should be adopted as a routine procedure in the laboratoryKeywords Candida species Etest Hemolysin Biofilm formation Proteinase phospholipase activity
Authors and Affiliations
Selvaraj Stephen, Pramodhinii S. , Shanmugam J. , Bhuvaneshwar D.
Keywords
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- EP ID EP473845
- DOI 10.18231/2394-5478.2018.0022
- Views 71
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