STUDIES ON THE MAJOR FACTORS AFFECTING IN VITRO MICROPROPAGATION OF TWO INTERGENERIC HYBRIDS FRAGARIA × POTENTILLA
Journal Title: Current Trends in Natural Sciences - Year 2012, Vol 1, Issue 1
Abstract
In order to establish the major factors affecting in vitro micropropagation of intergeneric hybrids Fragaria × Potentilla, respectively ‘Pink Panda’ and ‘Serenata’, basic culture media Murashige-Skoog (MS), Lee-Fossard (LF) and Knop, were supplemented with 6-benzylaminopurine (BAP), kinetine (Kin), indole-3-acetic acid (AIA), indole-3- butyric acid (AIB) and gibberellic acid (GA3), in different combination and concentration. In ornamental strawberry ‘Serenata’, which showed a genetic potential of shoot regeneration significantly higher compared with ‘Pink Panda’, a high multiplication rate associated with a high vigor of shoots was obtained on MS medium supplemented with 0.5 mg/l BAP + 0.1 mg/l AIB + 0.1 mg/l GA3. The same combination of growth regulators, added in MS medium in higher concentrations, namely 1.0 mg/l BAP + 0.2 mg/l AIB + 0.1 mg/l GA3 led to the highest rate of multiplication in ‘Pink Panda’ intergeneric hybrid of Fragaria × Potentilla.
Authors and Affiliations
Anca Nicoleta Sutan, A. Popescu, Valentina Isac
Keywords
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